A7080
Amphiregulin human
recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)
Synonyme(s) :
AREG, CRDGF, SDGF, AR
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About This Item
Produits recommandés
Source biologique
human
Niveau de qualité
Produit recombinant
expressed in E. coli
Pureté
≥97% (SDS-PAGE)
Forme
lyophilized powder
Puissance
10-100 ng/mL ED50/EC50
Poids mol.
~11 kDa
Conditionnement
pkg of 1 mg
Conditions de stockage
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
Technique(s)
cell culture | mammalian: suitable
Impuretés
endotoxin, tested
Numéro d'accès UniProt
Température de stockage
−20°C
Informations sur le gène
human ... AREG(374)
Actions biochimiques/physiologiques
Also known as keratinocyte autocrine factor (KAF); expression of amphiregulin mRNA has been found in numerous carcinoma cell lines and epithelial cells of several human tissues including colon, stomach, breast, ovary, and kidney.
Composants
Human amphiregulin is produced by a DNA sequence that encodes a 98 amino acid residue form of mature human amphiregulin. This corresponds to amino acid residues 101-198 in E. coli. Amphiregulin is a glycoprotein produced in response to treatment of the cell line MCF-7, a human breast carcinoma cell line, with PMA, a protein kinase C activator and potent tumor promoter.
Attention
This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.
Forme physique
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein.
Notes préparatoires
It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 10 μg/mL.
Remarque sur l'analyse
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is typically 5-15 ng/mL.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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