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93017

Sigma-Aldrich

SSC Buffer 20x

for Northern and Southern blotting, solution

Synonyme(s) :

20x SSC

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10 L
817.00 CHF

817.00 CHF

Date d'expédition estimée le02 avril 2025

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10 L
817.00 CHF

About This Item

Code UNSPSC :
12161700
Nomenclature NACRES :
NA.25

817.00 CHF

Date d'expédition estimée le02 avril 2025

Qualité

for molecular biology

Niveau de qualité

200

Gamme de produits

BioReagent

Forme

solution

Technique(s)

Southern blotting: suitable

Impuretés

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

pH

7.0±0.2

Densité

1.16 g/mL at 20 °C

Température de stockage

room temp

Description générale

SSC Buffer 20x Concentrate is a standard reagent in Southern and Northern blotting procedures. This concentrated formula can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Application

Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Caractéristiques et avantages

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations
  • Easily diluted to any concentration needed

Composants

SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
BCCL6351
BCCK4994
BCCH4851
BCCG6608
BCCG1453

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Guanghua Yang et al.
BMC biology, 11, 86-86 (2013-07-23)
Human pluripotent stem cells (hPSCs) hold great promise for applications in regenerative medicine. However, the safety of cell therapy using differentiated hPSC derivatives must be improved through methods that will permit the transplantation of homogenous populations of a specific cell
Sterghios A Moschos et al.
BMC genomics, 8, 240-240 (2007-07-21)
At present, nothing is known of the role of miRNAs in the immune response in vivo despite the fact that inflammation is thought to underlie multiple acute and chronic diseases. In these circumstances, patients are commonly treated with corticosteroids such

Protocoles

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Contenu apparenté

Water for Nucleic Acid Gel Electrophoresis

Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

Questions

  1. Hi how long time can I keep open sscx20 solution? What the expire time for this? Same question i have for ddulitions for this bbuffer, f.eks 2xssc?

    1 answer

    1. Provided the product is handled proprerly and not contaminated, it is quite stable. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

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