PF092

Fas Ligand Plus, Human, Recombinant

Fas Ligand Plus, Human, Recombinant, consists of amino acids 103-281 fused to a 26-amino acid linker and FLAG^®-tag at the N-terminus and expressed in HEK293 cells.

• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Produit recombinant: expressed in HEK 293 cells
• Niveau de qualité: 100
• Essai: ≥95% (SDS-PAGE)
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Recombinant, human Fas ligand (amino acids 103-281) fused to a 26-amino acid linker and FLAG^®-tag at the N-terminus and expressed in HEK293 cells. Glycosylation of recombinant human sFasL is similar or identical to natural human FasL. The molecular weight is ~32 kDa (nonglycosylated) and ~37-42 kDa (glycosylated) by SDS-PAGE under reducing conditions. The recombinant protein is produced in HEK293 cells. It does not require enhancer for activity. Useful for cytotoxicity assays.



Fas Ligand (FasL/APO-1L/CD95L) is a ~37-42 kDa type II transmembrane protein belonging to the TNF family. Interaction between FasL and Fas induces apoptosis of Fas-sensitive cells. FasL is implicated in CTL-mediated-killing, activation-induced cell death, creation of immune-privileged sites and tissue homeostasis. The extracellular domain of human FasL can be cleaved off by a metalloprotease, generating soluble FasL (sFasL). Circulating sFasL can be detected in serum of patients with melanoma and certain types of lymphoma.

Actions biochimiques/physiologiques

≥1 ng/ml kills Fas-sensitive cells

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from PBS.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Reconstitute in 50 µl PBS to yield a final stock concentration of 100 µg/ml. Further dilute in culture medium with 5% FCS immediately prior to use.

Autres remarques

Ashkenazi, A., and Dixit, V.M. 1998. Science281, 1305.
Nagata, S., 1997 Cell88, 355.
Hahne, M., et al. 1996. Science274, 1363.
Bellgrau, D., et al. 1995. Nature377, 630.
Lowin, B., et al. 1994. Nature370, 650.
Russell, J.H., et al. 1993. Proc. Natl. Acad. Sci. USA90, 4409.

Glycosylation of recombinant human sFasL is similar or identical to natural human FasL. The molecular weight is ~32 kDa (nonglycosylated) and ~35 kDa (glycosylated) by SDS-PAGE under reducing conditions. The recombinant protein is produced in HEK293 cells. It does not require enhancer for activity.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable