227036

Cholera Toxin

from Vibrio cholerae, ≥95% (SDS-PAGE), solid, A-B type toxin, Calbiochem

• Synonyme(s) : Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free
• Numéro CAS: 9012-63-9
• Numéro MDL: MFCD00130776
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.51

Propriétés

• product name: Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free, Cholera toxin Inaba 569B, CAS 9012-63-9, has a single A subunit surrounded by five polypeptides chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins.
• Niveau de qualité: 100
• Pureté: ≥95% (SDS-PAGE)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: do not freeze
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

Useful for studies involving adenylate cyclase, AMP, and related membrane-transport phenomena. Consists of a single A subunit surrounded by five polypeptide chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit. The B subunits are responsible for the attachment of the native toxin on the cell surface.

Useful for studies involving adenylate cyclase, AMP, and related membrane-transport phenomena. Consists of a single A subunit surrounded by five polypeptide chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit. The B subunits are responsible for the attachment of the native toxin on the cell surface..

Useful for tissue culture applications.

Actions biochimiques/physiologiques

Cell permeable: no

Primary Target
α-subunit of G-proteins

Product does not compete with ATP.

Reversible: no

Conditionnement

Please refer to vial label for lot-specific concentration.

yes

Avertissement

Toxicity: Highly Toxic (H)

Forme physique

Lyophilized from 200 mM NaCl, 50 mM Tris, 1 mM EDTA, pH 7.5.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 6 months at 4°C under sterile conditions.

Please refer to the vial label for lot-specific reconstitution. Note: cholera toxin tends to breakdown into its A and B subunits when the solution is frozen or with vigorous shaking during reconstitution, resulting in damage to the toxin protein and concomitant loss of biological activity. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that would lead to foaming. Heating of cholera toxin in excess of 40-50°C results in aggregation and subsequent breakdown into its A and B subunits.

Remarque sur l'analyse

Binding activity is assessed by hemagglutination assay using trisialoganglioside fixed sheep red blood cells.

single band by disc gel electrophoresis

Autres remarques

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Gilman, A.G. 1987. Annu. Rev.Biochem.56, 615.

Informations légales

Not available for sale outside of the United States.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS06
• Mention d'avertissement: Danger
• Mentions de danger: H300 + H310,H315
• Conseils de prudence: P262 - P264 - P270 - P280 - P301 + P310 - P302 + P352 + P310
• Classification des risques: Acute Tox. 1 Dermal - Acute Tox. 1 Oral - Skin Irrit. 2
• Code de la classe de stockage: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de danger pour l'eau (WGK): WGK 3