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SAE0069

Sigma-Aldrich

Cholera Toxin B subunit

≥95% (SDS-PAGE), lyophilized powder, enterotoxin

Synonyme(s) :

CTB, CTxB, Cholera enterotoxin B chain, Cholera enterotoxin gamma chain, Cholera enterotoxin subunit B, Choleragenoid, ToxB

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

product name

Cholera Toxin B subunit, recombinant, expressed in HEK 293 cells, subunit A free

Produit recombinant

expressed in HEK 293 cells

Niveau de qualité

200

Pureté

≥95% (SDS-PAGE)

Forme

lyophilized powder

Activité spécifique

(Binds to GM1 ganglioside. The binding EC50 is lower than 1 ug/ml)

Poids mol.

pentamer observed mol wt ~70 kDa by SDS-PAGE (non-reducing)
calculated mol wt 13 kDa
observed mol wt ~13 & 20 kDa by SDS-PAGE (reducing)

Solubilité

H2O: soluble 11 mg/mL

Numéro d'accès UniProt

P01556

Température de stockage

−20°C

Description générale

The advantage of this product over the native CTXB is that it has no traces of Vibrio cholera, and no contamination of the highly toxic Cholera toxin A subunit. This product is expressed in human HEK 293 cells as a C-terminally his-tagged protein with a calculated molecular mass of 13 kDa (amino acids Thr22-Asn124).
The protein is recombinant, and therefore has no contamination of the highly toxic Cholera toxin A subunit.
Under non-reducing conditions, it migrates as pentamer of ~70 kDa, consistent with the pentamerization of CTXB.

Application

Cholera Toxin B subunit has been used:
  • for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.
  • in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.

Forme physique

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Reconstitution

When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 1X PBS

Remarque sur l'analyse

Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with ≤1 μg of Cholera toxin B subunit per mL.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Si-Yuan Song et al.
Frontiers in neuroanatomy, 14, 600555-600555 (2020-12-18)
This study aimed to investigate the direct monosynaptic projections from cortical functional regions to the cerebrospinal fluid (CSF)-contacting nucleus for understanding the functions of the CSF-contacting nucleus. The Sprague-Dawley rats received cholera toxin B subunit (CB) injections into the CSF-contacting

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