LC/MS Extracted Ion Chromatogram of MSX, Sulfone, and Sulfoxide on Ascentis® Express OH5 (HILIC Mode)
CONDITIONS
column
Ascentis Express OH5, 10 cm x 3 mm I.D., 2.7 μm particles (53769-U)
mobile phase
[A] 0.1% formic acid, pH 3.5 with ammonium formate; [B] acetonitrile; (25:75, A:B)
flow rate
0.4 mL/min
pressure
1350 psi (93 bar)
column temp.
ambient
detector
ESI (+), scan m/z 150-300
injection
5 μL
sample
10 μg/mL in 90% methanol
Beschreibung
Hinweis zur Analyse
This figure shows the extracted ion currents for methionine sulfoximine-related compounds on Ascentis Express OH5. The OH5 shows excellent peak shape compared to traditional HILIC on bare silica.
Rechtliche Hinweise
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany