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HPLC Analysis of Taxols on Ascentis® Express F5

HPLC Analysis of Taxols on Ascentis® Express F5 application for HPLC

Materialien

Analytische Säule

Produkt-Nr.
Beschreibung
Preisangaben

Ascentis® Express F5, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

Standard

Produkt-Nr.
Beschreibung
Preisangaben

Baccatin III

≥95% (HPLC)

Kephalomannin

≥97% (HPLC), solid

Paclitaxel-verwandte Verbindung B

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)

mobile phase

[A] water; [B] acetonitrile; (60:40, A:B)

flow rate

0.3 mL/min

column temp.

30 °C

detector

UV, 227 nm

injection

2 μL

sample

25 mg/L in 70:30, water:methanol

Beschreibung

Hinweis zur Analyse

This application demonstrates the suitability of Ascentis Express F5 for the efficient separation of taxols.

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany