HPLC Analysis of Phenylacetic Acid on Ascentis® C18
CONDITIONS
column
Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)
mobile phase
[A] 20 mM phosphoric acid; [B] acetonitrile; (75:25, A:B)
flow rate
1.0 mL/min
column temp.
35 °C
detector
UV, 215 nm
injection
5 μL
sample
50 μg/mL in water: acetonitrile: methanol (75: 20: 5)
Beschreibung
Hinweis zur Analyse
This application demonstrates the suitability of Ascentis C18 for the analysis of phenylacetic acid.
Rechtliche Hinweise
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany