ZRB1579

Anti-ISL-1 Antibody, clone 1B11 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): Insulin gene enhancer protein ISL-1;Islet-1
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Rekombinant: expressed in HEK 293 cells
• Konjugat: unconjugated
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 1B11, recombinant monoclonal
• Beschreibung: recombinant, expressed in HEK 293 cells
• Produktlinie: ZooMAb^® learn more
• Form: lyophilized
• Mol-Gew.: calculated mol wt 39.04 kDa; observed mol wt ~42 kDa
• Aufgereinigt durch: using Protein A
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): canine, bovine, rat, monkey, mouse
• Verpackung: antibody small pack of 25 μL
• Grünere Alternativprodukt-Eigenschaften: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Erweiterte Validierung: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Methode(n): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotyp: IgG
• Epitopsequenz: C-terminal
• Protein-ID-Hinterlegungsnummer: NP_002193
• UniProt-Hinterlegungsnummer: P61371
• Grünere Alternativprodukt-Kategorie: , Aligned
• Versandbedingung: ambient
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spezifität

Clone 1B11 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Insulin gene enhancer protein ISL-1. It targets an epitope within 21 amino acids within the LIM binding domain in the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the LIM binding domain in the C-terminal region of human Insulin gene enhancer protein ISL-1.

Anwendung

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in human pancreas tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected ISL-1 in human pancreas tissue sections.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ISL-1 in SH-SY5Y cell lysate.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected ISL-1 in SH-SY5Y cells.

Affinity Binding Assay: A representative lot of this antibody bound ISL-1 peptide with a KD of 5.6 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Zielbeschreibung

Insulin gene enhancer protein ISL-1 (UniProt: P61371; also known as Islet-1, ISL-1) is encoded by the ISL1 gene (Gene ID: 3670) in human. ISL-1 is a member of the LIM homeodomain family of transcription factors that contain two tandemly repeated cysteine/histidine-rich LIM domains that are involved in protein-protein interactions. It is a DNA-binding transcriptional activator that recognizes and binds to the consensus octamer binding site 5′-ATAATTAA-3′ in promoter of target genes. Its expression is observed in in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain. It is shown to play a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. It is also involved in the specification of motor neurons in cooperation with LHX3 and LDB1. ISL-1 is also established as an important stem cell marker for cardiac progenitor cells in the developing heart. It is reported to bind to compacted chromatin and works in concert with the Brg1-Baf60c-based SWI/SNF complex to promote cardiac lineage-specific alterations in the chromatin landscape of genes that function in cardiac progenitors and cardiomyocyte structural genes. It is also expressed in a subpopulation of striatal progenitor cells that give rise to striatal neurons that belong to the striatonigral pathway. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gao, R., et al. (2019). Cell Res. 29(6); 486-501; Ehrman, LA., et al. (2013). Proc. Natl. Acad. Sci. USA. 110(42); E40276-E4035).

Physikalische Form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Rekonstituierung

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Rechtliche Hinweise

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 13 - Non Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable