T0910

Thioredoxin from Escherichia coli

recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

• CAS-Nummer: 52500-60-4
• MDL-Nummer: MFCD00132423
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: Escherichia coli
• Qualitätsniveau: 200
• Rekombinant: expressed in E. coli
• Assay: ≥90% (SDS-PAGE)
• Form: essentially salt-free, lyophilized powder
• Spezifische Aktivität: ≥3 units/mg protein
• Mol-Gew.: 11.7 kDa
• Methode(n): activity assay: suitable
• Löslichkeit: water: 1.90-2.10 mg/mL, clear, colorless
• Protein-ID-Hinterlegungsnummer: M26133
• UniProt-Hinterlegungsnummer: P0AA25
• Anwendung(en): cell analysis
• Lagertemp.: −20°C
• Angaben zum Gen: Escherichia coli K12 ... trxA(948289)

Beschreibung

Allgemeine Beschreibung

Research area: Cell Signaling

Thioredoxin from Escherichia coli is a monomeric, globular protein. It has a central β-sheet surrounded by α-helices.[1]

Anwendung

Thioredoxin from Escherichia coli has been used in thioredoxin reductase assay, peroxidase-coupled thioredoxin system assay and peroxiredoxin assay.[2][3][4] It has also been used to study its effects on photoreceptor death in a mouse model of retinitis pigmentosa (RP) and its potential to modify retinal oxidative stress, glial changes, and vascular changes during the progression of the disease.[5]

Biochem./physiol. Wirkung

Mediates the reduction of disulfide bonds in proteins.

Thioredoxin from Escherichia coli catalyzes REDOX reactions and has a role in various biological processes. It modulates the structure and activity of many proteins. The enzyme thioredoxin reductase catalyzes the reduction of thioredoxin. Thioredoxin also has a role in oxidative stress response.[1][6][7]Thioredoxin, known as both adult T cell leukemia-derived factor (ADF) and a cellular redox enzyme, functions as a cytokine or chemokine, facilitating cell-to-cell communication.[8] Additionally, it plays a role in regulating cell growth and apoptosis.[9]

Sonstige Hinweise

GenBank^® Accession Number M26133.

Einheitendefinition

One unit will cause a ΔA₆₅₀ of 1.0 in 1 min at 25 °C in the insulin reduction assay.

Rechtliche Hinweise

GenBank is a registered trademark of United States Department of Health and Human Services

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)