Direkt zum Inhalt
Merck
Alle Fotos(2)

Dokumente

CS0170

Sigma-Aldrich

Thioredoxin Reductase Assay Kit

1 kit sufficient for 100 assays (1 mL)

Synonym(e):

Thioredoxin Reductase Activity Kit

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(2)

About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Qualitätsniveau

200

Verwendung

 kit sufficient for 100 assays (1 mL)

Versandbedingung

dry ice

Lagertemp.

−20°C

Angaben zum Gen

human ... TXNRD1(7296) , TXNRD2(10587) , TXNRD3(114112)

Verwandte Kategorien

Allgemeine Beschreibung

The Thioredoxin Reductase Assay Kit uses a colorimetric assay for the determination of thioredoxin reductase activity in mammalian cells and tissues. It is based on the reduction of 5,5′-dithiobis(2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) that produces a strong yellow color that is measured at 412 nm.

DTNB + NADPH + H+ ↔ 2 TNB + NADP+

Anwendung

Using post nuclear extracts of rat liver, the kit shows linearity up to 0.25 units per mL. In this preparation, ~20% of the activity is due to non-specific reductase activity (refractory to the TrxR inhibitor).

Leistungsmerkmale und Vorteile

  • An easy and simple method
  • The kit contains all the reagents required for a colorimetric assay of mammalian thioredoxin reductase
  • The kit includes an inhibitor solution for specific inhibition of mammalian thioredoxin reductase
  • The kit has been tested with heart muscle, as well as lysates from cell lines such as HeLa, A549, Jurkat, U937, A431, COS, CHO, and NIH 3T3 cells

Nur Kit-Komponenten

Produkt-Nr.
Beschreibung
  • Assay Buffer 5x for Thioredoxin Reductase 30 mL
  • Thioredoxin Reductase 10 μg
  • Thioredoxin Reductase Inhibitor Solution .05 mL
  • 5,5'-Dithiobis(2-nitrobenzoic) acid (DNTB) 150 mg
  • NADPH 25 mg
  • Dimethyl Sulfoxide (DMSO) 7.5 mL

Piktogramme

Health hazard
GHS08

Signalwort

Warning

H-Sätze

H361

Gefahreneinstufungen

Repr. 2

Lagerklassenschlüssel

10 - Combustible liquids

Flammpunkt (°F)

188.6 °F

Flammpunkt (°C)

87 °C

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Tessa D Epstein et al.
ACS infectious diseases, 5(10), 1682-1687 (2019-09-06)
Helicobacter pylori (H. pylori) infection poses a worldwide public health crisis, as chronic infection is rampant and can lead to gastric ulcers, gastritis, and gastric cancer. Unfortunately, frontline therapies cause harmful side effects and are often ineffective due to antibiotic resistance.
Matthew T Harper
Platelets, 30(1), 98-104 (2017-12-02)
Platelets are central to normal hemostasis and must be tightly controlled to prevent thrombosis. However, drug treatments that also affect platelets could lead to unwanted side effects on hemostasis or thrombosis. In this study, the effect of auranofin on platelets
Francesca Magherini et al.
Oncotarget, 9(46), 28042-28068 (2018-07-03)
Au(NHC) and Au(NHC)2, i.e. a monocarbene gold(I) complex and the corresponding bis(carbene) complex, are two structurally related compounds, endowed with cytotoxic properties against several cancer cell lines. Herein, we explore the molecular and cellular mechanisms at the basis of their
Melissa A Fath et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 17(19), 6206-6217 (2011-08-17)
Cancer cells (relative to normal cells) show increased steady-state levels of hydroperoxides that are compensated by increased glucose and hydroperoxide metabolism. The current study determined whether inhibitors of glucose and hydroperoxide metabolism could induce chemoradiosensitization by enhancing oxidative stress in
Giulia Gorini et al.
Biomedicines, 9(8) (2021-08-28)
Au2phen ((2,9-dimethyl-1,10-phenanthroline)2Au2(µ-O)2)(PF6)2 and Auoxo6 ((6,6'-dimethyl-2,2'-bipyridine)2Au2(µ-O)2)(PF6)2 are two structurally related gold(III) complexes that were previously reported to display relevant and promising anticancer properties in vitro toward a large number of human cancer cell lines. To expand the knowledge on the molecular
Alle zugehörigen Dokumente anzeigen

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.