SRP8043

APRIL, Soluble mouse

recombinant, expressed in HEK 293 cells, FLAG^® tagged, >95% (SDS-PAGE)

• Synonym(e): ACRP30headless:A-proliferation-inducing ligand, ACRP30headless:APRIL, ACRP30headless:CD256, TNFSF10
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: mouse
• Rekombinant: expressed in HEK 293 cells
• Markierung: FLAG^® tagged
• Assay: >95% (SDS-PAGE)
• Form: lyophilized
• Mol-Gew.: ~34 kDa by SDS-PAGE
• Verpackung: pkg of 10 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Verunreinigungen: <0.02 EU/μg endotoxin, tested
• Farbe: white
• UniProt-Hinterlegungsnummer: Q9D777
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... Tnfsf13(69583)

Beschreibung

Allgemeine Beschreibung

APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophage-mediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. APRIL is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the N-terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells.

Physikalische Form

Lyophilized from PBS.

Rekonstituierung

Reconstitute with 100 μL deionized water.

Sonstige Hinweise

The extracellular domain of mouse APRIL (aa 88-232) is fused at the N-terminus to mouse ACRP30headless (aa 18-110) and a FLAG^®-tag.

Rechtliche Hinweise

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable