SRP3316

LIF human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(e): D factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor (MLPLI)
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Rekombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Form: lyophilized
• Mol-Gew.: 19.6 kDa
• Verunreinigungen: endotoxin, tested
• UniProt-Hinterlegungsnummer: P15018
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... LIF(3976)

Beschreibung

Allgemeine Beschreibung

Leukemia inhibitory factor (LIF) is a secreted pleiotropic glycoprotein which is a member of the gp130 signaling of interleukin-6 (IL-6) family cytokines.[1][2] This protein was originally identified as a protein that prevented the growth of a leukemic cell line.[3]
Recombinant human LIF is a 19.6 kDa protein containing 180 amino acids residues including three disulfide bonds.

Biochem./physiol. Wirkung

Leukemia inhibitory factor (LIF) suppresses the differentiation of myoblasts, and inhibits the activation of caspase-3 and blocks the reduction in Bcl-xL (B-cell lymphoma-extra large) levels, thus, preventing doxorubicin-induced apoptosis. Along with transforming growth factor β2 (TGFβ2)/fibroblast growth factor 2 (FGF2), LIF regulates nephrogenesis. It facilitates tubular regeneration following acute kidney failure. It plays a crucial role in neuroprotective pathways induced by preconditioning-induced oxidative stress.[1] LIF is capable of maintaining the totipotency of embryonic stem cells.[3] It is also involved in controlling the differentiation of embryonic stem cells, hematopoietic and neuronal cells.[2]

Physikalische Form

Lyophilized with no additives

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable