The supplied amount of protein is 10 μg. The concentration of the protein is approximately 0.1 μg/μL, but may vary slightly from lot to lot. This minimum purity is 70% as determined by densitometry. The PP2Aa molecular weight is approximately 38 kDa and the PP2R1A molecular weight is approximately 65 kDa as shown in the image below:
SRP0736
PP2A ALPHA/PPP2R1A Complex, Active
recombinant
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10 μG
CHF 445.00
CHF 445.00
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10 μG
CHF 445.00
About This Item
UNSPSC-Code:
12352202
NACRES:
NA.77
CHF 445.00
Check Cart for Availability
Empfohlene Produkte
Rekombinant
expressed in baculovirus infected Sf9 cells
Assay
70-95% (SDS-PAGE)
Form
frozen liquid
Spezifische Aktivität
3400-4600 nmol/min·mg (BIOMOL Green)
Mol-Gew.
~38 kDa
~65 kDa
Konzentration
0.1 μg/μL
Hinterlegungsnummer
NM_002715
NM_014225
Versandbedingung
dry ice
Lagertemp.
−70°C
Angaben zum Gen
human ... PP2A(5524) , PPP2R1A(5518)
Anwendung
Useful for Phosphatase assay and Western blot.
Biochem./physiol. Wirkung
Recombinant full length human PP2Aalpha and human PPP2R1A were expressed by Baculovirus in Sf9 insect cells using an N-terminal His tag. PP2Aalpha is also known as Protein phosphatase 2A active subunit. PP2A is one of the four major Ser/Thr phosphatases and is a human tumor suppressor that inhibits cellular transformation. PP2A alpha gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase-2A (1). Protein phosphatase 2A holoenzyme is a heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. PTP2A alpha can alter the substrate specificity of PP2A leading to enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity (2).
Rekonstituierung
Formulated in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.
Lagerung und Haltbarkeit
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Lagerklassenschlüssel
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Lot/Batch Number
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What is the measured mass of protein in this product?
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