SRP0211

PTP-D2/PTPN14 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonym(e): Cytoskeletal-associated protein tyrosine phosphatase, PTP36, PTPN14, Pez, Protein tyrosine phosphatase, non-receptor type 14
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in E. coli
• Assay: ≥90% (SDS-PAGE)
• Form: aqueous solution
• Mol-Gew.: 61 kDa
• Verpackung: pkg of 20 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Konzentration: >0.02 mg/mL
• NCBI-Hinterlegungsnummer: NM_005401
• UniProt-Hinterlegungsnummer: Q15678
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... PTPN14(5784)

Beschreibung

Allgemeine Beschreibung

Catalytic domain, (amino acids 817-1188), (Gene bank accession number NM_005401) with N-terminal GST tag, MW = 61 kDa, expressed in an E. coli expression system.

Anwendung

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Einheitendefinition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.