SAE0196

Achromopeptidase from bacteria

free of DNA contaminants, suitable for Microbiome research

• Synonym(e): Bacterial Protease
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: bacterial
• Qualitätsniveau: 200
• Form: lyophilized powder
• Spezifische Aktivität: ≥1000 U/mg
• Leistungsmerkmale: DNA free
• Methode(n): DNA extraction: suitable
• Eignung: suitable for cell lysis
• Anwendung(en): cell analysis
• Versandbedingung: ambient
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme, as well as Gram-negative organisms. Achromopeptidase has been shown to contain two bacteriolytic proteases. This product is free of detectable microbial DNA and is suitable for microbiome research

Anwendung

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme, as well as Gram-negative organisms. Achromopeptidase has been shown to contain two bacteriolytic proteases.

The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. This product undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.

Biochem./physiol. Wirkung

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. Optimum pH is 8.5-9.

Leistungsmerkmale und Vorteile

Contains two bacteriolytic proteases for efficient lysis of Gram-positive bacteria and Gram-negative organisms

Free of detectable microbial DNA, ensuring the accuracy of metagenomics and other culture-independent sequencing methods

Undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA

Optimum pH of 8.5-9 allows for use in a wide range of microbiome studies

Einheitendefinition

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable