SAB4200277

Anti-RCC1 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(e): Anti-CHC-1, Anti-CHC1, Anti-Chromosome Condensation 1, Anti-Regulator of Chromatin Condensation 1
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: ~45 kDa
• Speziesreaktivität: human
• Konzentration: ~1.0 mg/mL
• Methode(n): immunoprecipitation (IP): 2.5-5.0 μg using lysates of human HEK-293 cells.; indirect immunofluorescence: 1.0-2.0 μg/mL using paraformaldehyde fixed HeLa cells.; western blot: 0.5-1.0 μg/mL using lysates of HeLa cells
• UniProt-Hinterlegungsnummer: P18754
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... RCC1(1104)

Beschreibung

Allgemeine Beschreibung

Regulator of chromosome condensation 1 (RCC1) is a nuclear chromatin-bound protein and a guanine nucleotide exchange factor (GEF) for the nuclear Ras-related nuclear (Ran) protein. It consists of seven homologous repeats of 51−68 amino acid residues termed RCC1-like domains (RLDs), which adopt a seven-bladed β-propeller fold 2 and an amino terminal tail extension. The RCC1 gene is mapped to human chromosome 1p35.3.(4)

Spezifität

Anti-RCC1 (N-terminal) recognizes human RCC1.

Immunogen

synthetic peptide corresponding to a sequence at the N- terminal region of human RCC1, conjugated to KLH. The corresponding sequence differs by one amino acid in rat or mouse.

Anwendung

Anti-RCC1 (N-terminal) antibody produced in rabbit is suitable for:

• immunoblotting
• immunofluorescence
• immunoprecipitation

Biochem./physiol. Wirkung

Regulator of chromosome condensation 1 (RCC1) protein is an important cell cycle regulator. It is associated dynamically with chromatin through binding to histones H2A and/or H2B in a Ras-related nuclear (Ran) regulated manner. RCC1 has been shown to play essential roles in nuclear transport, spindle organization and nuclear envelop formation. α-N-methylation of RCC1 is essential for strong attachment of RCC1 to chromatin during normal mitosis.

Physikalische Form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable