R1158

Ribonuclease Inhibitor

recombinant, expressed in E. coli, 20-40 units/μL

• Synonym(e): RNAse inhibitor
• MDL-Nummer: MFCD00132187
• UNSPSC-Code: 12352200
• NACRES: NA.52

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 300
• Qualität: for molecular biology
• Form: buffered aqueous solution
• Mol-Gew.: ~50 kDa
• Konzentration: 20-40 units/μL
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is derived from E. coli which expresses portions of the human placental ribonuclease inhibitor. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes. The pH range for inhibition is pH 5.5–9 (highestinhibition at pH 7–8).

Anwendung

Ribonuclease Inhibitor is suitable for use in useful for in vitro inhibition of ribonucleases, including procedures like:

• cDNA synthesis from mRNA
• reverse transcriptase polymerase chain reaction (RT-PCR)
• in vitro transcription and translation
• mRNA protection from degradation[1]
• formaldehyde cross-linked lncRNA purification[2]
• RNA-fluorescence in situ hybridization (FISH)[3]

Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.

Biochem./physiol. Wirkung

Inhibits RNase by forming a tight, non-covalent 1:1 complex.

Komponenten

Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.

Einheitendefinition

One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.

Physikalische Form

Solution in 20 mM HEPES-KOH, pH 7.6, 50 mM KCl, 8 mM DTT and 50% glycerol.

Sonstige Hinweise

• Denaturing conditions (i.e., urea or temperatures ≥50 °C) should be avoided as they may cause a release of active ribonuclease from the complex.
• This product is for R&D use only, not for drug, household, or other uses.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable