N4787

Nek2, Active human

recombinant, expressed in E. coli, ≥85% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(e): Never In Mitosis Gene A-Related Kinase 2, Nima-Related Kinase 2
• MDL-Nummer: MFCD07370971
• UNSPSC-Code: 51111800
• NACRES: NA.32

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 200
• Assay: ≥85% (SDS-PAGE)
• Form: buffered aqueous glycerol solution
• enzyme activity: ≥10 units/mg protein
• Mol-Gew.: protein apparent mol wt ~80 kDa
• UniProt-Hinterlegungsnummer: P51955
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... NEK2(4751)

Beschreibung

Anwendung

Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

Biochem./physiol. Wirkung

Nek2 (nimA-related kinase 2) is a cell cycle-regulated protein kinase with maximal activity at the onset of mitosis that localizes to the centrosome.

Einheitendefinition

One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP

Physikalische Form

Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable