The product MAK106 uses linear regression fitting. The standard curve is prepared based on the donor particle concentration indicated on the label, which is batch-specific. The standard curve in table 2 is representative and for example purposes only. It's important to note that the concentrations may vary. The pmole values are calculated through dimension analysis using the amount of donor particle solution used and any subsequent dilution. For example, the most concentrated standard can be calculated by considering the volume of the donor particle. In this case, the most concentrated standard has 130 pmoles. Each subsequent dilution is two times less concentrated, resulting in concentrations of 65, 32.5, 16.25, and 8.1 pmoles.
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MAK106
CETP Activity Assay Kit
Supplied by Roar Biomedical, Inc.
Synonym(e):
Cholesteryl ester transfer protein activity assay kit
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1 KIT
CHF 669.00
About This Item
UNSPSC-Code:
12161503
NACRES:
NA.84
Empfohlene Produkte
Verwendung
sufficient for 100 fluorometric tests
Anwendung(en)
pharmaceutical
Nachweisverfahren
fluorometric
Relevante Krankheit(en)
cardiovascular diseases
Lagertemp.
2-8°C
Angaben zum Gen
human ... CETP(1071)
Verwandte Kategorien
Allgemeine Beschreibung
Cholesteryl ester transfer protein (CETP) is present in normal human plasma. The protein transfers neutral lipids from high density lipoproteins (HDL) to very low density lipoprotein (VLDL) and low density lipoprotein (LDL). CETP plays an important role in lipoprotein metabolism and influences the reverse cholesterol transport pathway. The method is useful for measuring CETP activity in plasma or serum in all species that express CETP.
Anwendung
Cholesteryl ester transfer protein (CETP) activity Assay Kit has been used for measurement of CETP activity in the plasma samples.[1]
Leistungsmerkmale und Vorteile
Compatible with high-throughput handling systems.
Eignung
Suitable for the detection of cholesteryl ester transfer protein (CETP) in plasma / serum (fresh or frozen), recombinant or purified CETP.
Prinzip
The CETP Activity Assay Kit uses a proprietary substrate that enables the detection of CETP-mediated transfer of neutral lipid from the substrate to a physiological acceptor. The transfer activity results in an increase in fluorescence intensity (λEx=535 nm/λEm=587 nm). In a total volume of 200 μL, the assay is linear from 0.2 to 0.8 μL of normal human plasma. Assay results not affected by endogenous plasma HDL, LDL or VLDL concentrations.
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10 - Combustible liquids
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What type of regression fitting is used for the product MAK106, and what is the method for calculating the pmoles shown in the typical standard curve in table 2?
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