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Merck

M1321

Sigma-Aldrich

Monoclonal Anti-Maltose Binding Protein in Maus hergestellte Antikörper

clone MBP-17, purified immunoglobulin, buffered aqueous solution

Synonym(e):

Anti-MBP

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200 μL
CHF 476.00

CHF 476.00


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200 μL
CHF 476.00

About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.46

CHF 476.00


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Biologische Quelle

mouse

Konjugat

unconjugated

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

MBP-17, monoclonal

Form

buffered aqueous solution

Konzentration

~2 mg/mL

Methode(n)

dot blot: suitable
indirect ELISA: suitable
western blot: 0.05-0.1 μg/mL using purified recombinant MBP.

Isotyp

IgG1

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

Monoclonal Anti-Maltose Binding Protein (MBP) (mouse IgG1 isotype) is derived from the hybridoma MBP-17 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified recombinant MBP fusion protein.
The antibody recognizes native as well as denatured-reduced forms of purified MBP and MBP fusion proteins.

Immunogen

Purified, recombinant MBP fusion protein.

Anwendung

Monoclonal Anti-Maltose Binding Protein antibody produced in mouse has been used in:
  • immunoblotting
  • dot blot
  • luminometric immunoassay
  • enzyme linked immuno sorbent assay (ELISA)

Monoclonal Anti-Maltose Binding Protein antibody produced in mouse was used in agarose binding assay for the detection of activation-induced cytidine deaminase-MBP complex.

Biochem./physiol. Wirkung

Maltose binding protein (MBP) is a periplasmic binding protein that is important for nutrient uptake and chemotaxis of Escherichia coli.
Maltose binding protein (MBP) tag creates a stable fusion product, that does not appear to interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. It facilitates the detection, isolation and purification of the proteins.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Andrew C Miklos et al.
PloS one, 8(10), e74969-e74969 (2013-10-15)
Cellular signaling involves a cascade of recognition events occurring in a complex environment with high concentrations of proteins, polysaccharides, and other macromolecules. The influence of macromolecular crowders on protein binding affinity through hard-core repulsion is well studied, and possible contributions
Wubei Zong et al.
The New phytologist, 229(3), 1635-1649 (2020-10-23)
Rice (Oryza sativa) is a short-day (SD) plant originally having strong photoperiod sensitivity (PS), with SDs promoting and long days (LDs) suppressing flowering. Although the evolution of PS in rice has been extensively studied, there are few studies that combine
Steffen Frey et al.
PloS one, 10(4), e0125099-e0125099 (2015-04-30)
During autophagy, members of the ubiquitin-like Atg8 protein family get conjugated to phosphatidylethanolamine and act as protein-recruiting scaffolds on the autophagosomal membrane. The Atg4 protease produces mature Atg8 from C-terminally extended precursors and deconjugates lipid-bound Atg8. We now found that
A rapid solubility-optimized screening procedure for recombinant subtilisins in E. coli
Bjerga GEK, et al.
Journal of Biotechnology, 222, 38-46 (2016)
Arturo Vera Rodriguez et al.
The Journal of cell biology, 218(6), 2006-2020 (2019-04-27)
Cleavage of affinity tags by specific proteases can be exploited for highly selective affinity chromatography. The SUMO/SENP1 system is the most efficient for such application but fails in eukaryotic expression because it cross-reacts with endogenous proteases. Using a novel selection

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