GE28-9269-73

HiScreen^™ MabSelect^™

Cytiva 28-9269-73

• UNSPSC-Code: 13111000
• NACRES: NA.56

Eigenschaften

• ligand: recombinant protein A (E. coli)
• Verpackung: pkg of 1 ea
• Hersteller/Markenname: Cytiva 28-9269-73
• Lagerbedingungen: (20% Ehtanol); (20% Ethanol)
• Parameter: 44 psi
• Bettgröße: 7.7 mm × 100 mm
• Bettvolumen: 4.7 mL
• Säule ID: 7.7 mm
• Matrix: highly cross-linked agarose
• Durchschnittliche Teilgröße: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 2-12
• working range: 3-10
• Kapazität: ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
• Eignung: suitable for bioprocess medium
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.

HiScreen^™ MabSelect^™ is prepacked with MabSelect^™ and is part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening for capture of monoclonal antibodies (MAbs).

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Anwendung

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen^™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up.

If necessary, two columns can easily be connected in series to give a bed height of 20 cm.

Media prepacked in HiScreen^™ MabSelect^™ columns are also available in other formats and as bulk packs, which makes it possible to use the same media for development work, pilot studies, and routine production.

Leistungsmerkmale und Vorteile

• MabSelect^™ is a BioProcess medium for capture of MAbs from large sample volumes.
• Prepacked, ready-to-use columns for convenient process development.
• 10 cm bed height of HiScreen^™ columns is designed to allow method optimization and parameter screening.
• Easily connected in series to achieve 20 cm bed height.
• Small bed volume gives fast results and minimal sample/buffer consumption.
• Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

HiScreen is a trademark of Cytiva

MabSelect is a trademark of Cytiva

Sicherheitshinweise

• Piktogramme: GHS02
• Signalwort: Warning
• H-Sätze: H226
• P-Sätze: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Lagerklassenschlüssel: 3 - Flammable liquids