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Merck

F0926

Sigma-Aldrich

Fetales Kälberserum

USDA approved, sterile-filtered, suitable for cell culture

Synonym(e):

FBS, FCS, Seren, Serum

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352207
NACRES:
NA.71
Biologische Quelle:
bovine fetus
Sterilität:
sterile-filtered
Versandbedingung:
dry ice

Biologische Quelle

bovine fetus

Qualitätsniveau

Sterilität

sterile-filtered

Produktlinie

FBS Classic

Qualität

USDA approved

Zusammensetzung

Hemoglobin, ≤25 mg/dL

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

≤10 EU/mL endotoxin

Versandbedingung

dry ice

Lagertemp.

−20°C

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Allgemeine Beschreibung

Fetal bovine serum (FBS) or fetal calf serum is the blood fraction remaining after the natural coagulation of blood. It comes from the blood drawn from a bovine fetus via a closed system of collection at the slaughterhouse. It is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells.

Anwendung

FBS sourced from USDA approved non-USA countries is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factors to cells in culture.

Fetal Bovine Serum has been used for the following cell cultures:
  • Primary Culture Human Macrophages (mononuclear cells were re-suspended in Roswell Park Memorial Institute (RPMI) medium containing fetal bovine serum (FBS) along with other components).
  • Tissue Culture (MEFs were cultured in DMEM supplemented with FBS along with components).
  • Isolation and 3D culture of primary mammary epithelial organoids
  • Cell culture and medium (HUVECs were grown in Endothelial Cell Growth Medium-2 supplemented with FBS and growth factors)

Eignung

USDA Approved Sourcing refers to FBS produced from blood collected in countries outside of the United States that have been approved by the USDA (United States Department of Agriculture) for export of beef products into the United States. Eligible countries for export into the U.S. include Australia, Canada, Chile, Costa Rica, Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay. Foreign serum is tested to ensure the absence of exotic viruses before release by the USDA.

Hinweis zur Analyse

  • Endotoxin- und Hämoglobin-getestet
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves


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Artikel

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.

Protokolle

This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

Verwandter Inhalt

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Explore our portfolio of fetal bovine serum (FBS) and determine the right FBS for your lab needs and budget. Request a serum sample.

Entdecken Sie unser Angebot an fötalem Rinderserum (FBS) und bestimmen Sie das richtige FBS für Ihre Laboranforderungen und Ihr Budget. Serumprobe bestellen

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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