CS0004

Nuclease mikrokokkal aus Staphylococcus aureus

With reaction buffer and BSA solution

• Synonym(e): Endonuclease mikrokokkal, MNase, Micrococcal Endonuclease
• CAS-Nummer: 9013-53-0
• UNSPSC Code: 12352202
• NACRES: NA.77
• EG-Nummer: 3.1.31.1 (BRENDA, IUBMB)
• MDL number: MFCD00131731
• Biological source: Staphylococcus aureus

Eigenschaften

• biological source: Staphylococcus aureus
• form: liquid
• shipped in: wet ice
• storage temp.: −20°C

Beschreibung

General description

Nuclease Micrococcal (MNase) is the extracellular nuclease of Staphylococcus aureus. The enzyme is an endo-exonuclease .This Endo-exonuclease preferentially digests single-stranded nucleic acids. Thus, nuclease micrococcal activity results in the production of mononucleotides and oligonucleotides with terminal 3′-phosphates. Nuclease Micrococcal is also active against double-stranded DNA and RNA and all sequences will be ultimately cleaved.
Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes

Application

• Chromatin Immuno-Percipitation (ChIP) analysis
• Nucleosome preparation
• Removal of nucleic acids present in protein preparation
• Reduction of viscosity of cell lysates during non-mechanical cell lysis preparation
• RNA sequencing and transcriptomic profiling

Biochem/physiol Actions

Hydrolysiert 5′-Phosphodiester-Bindungen der DNA.

Other Notes

Hinweis: Eine μmolare Einheit = ca. 85 A₂₆₀-Einheiten. Dabei entspricht eine A₂₆₀-Einheit einer ΔA₂₆₀-Ablesung von 1,0 nach 30 Minuten bei einem pH-Wert von 8,8 und 37 °C in einem Reaktionsvolumen von 3,55 ml. Lichtweg = 1 cm.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3