CSE-119

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: fish unknown/unspecified
• Verpackung: tube of 5 μg 95122019-DNA-5UG; pkg of vial of cells 95122019-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Diploid and modal no. 60
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Fish Coho salmon fibroblast

Beschreibung der Zelllinie

The fish cell line CSE-119 was initiated in 1963 from pooled embryos of Coho salmon (Oncorynchus kisutch). CSE-119, also known as Coho salmon embryo-119, are susceptible to infectious pancreatic necrosis virus (IPNV) but show a lack of sensitivity to infection with viral haemorrhagic septicaemia virus (VHSV) or infectious haematopoietic necrosis virus (IHNV). This might be correlated to the natural resistance to infectious haemotopoietic necrosis in this species. For replication of salmonid viruses CSE-119 cells should be incubated at 18°C.

Anwendung

Isolation of viruses from clinical samples, virus propagation

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 15-21°C; split every 10-14 days; requires a new flask every passage. Fish cell lines detach easily during transit if the culture is too young. After resuscitation spli

Sonstige Hinweise

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr J L Fryer, Department of Microbiology, Oregon State University,Corvallis