74 BLCL human cell line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., IHW Number: SLE, 94031612

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human blood
• Verpackung: tube of 5 μg 94031612-DNA-5UG; pkg of vial of cells 94031612-1VL
• Ethnizität: Caucasian
• Geschlecht: female
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Lymphoblastoid
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice

Beschreibung

Beschreibung der Zelllinie

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA-Profil

Not specified

Serologisches Profil

HLA A: 2 0
HLA C: 6 7
HLA B: 7 50
HLA DR: 2 7
HLA DQ: 1 2

Nährmedium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subkultur-Routine

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.