RENT tsS1.1

92092515, rat endometrium, Cuboidal

• UNSPSC-Code: 41106514

Eigenschaften

• product name: RENT tsS1.1, 92092515
• Biologische Quelle: rat endometrium
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Cuboidal
• Produkte: Not specified
• Rezeptoren: Glucocorticoid
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat Sprague-Dawley, primary endometrial, SV40 T antigen transformed

Beschreibung der Zelllinie

Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. The cells carry vimentin, CSF-1 (at 39°C), desmin, collagen type III and connexin 43 markers. The transforming agent is a ts mutant of SV 40 T-antigen (tsA58).

Nährmedium

DMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Doubling time 48 hours. Cells show continuous growth at 34°C and growth arrest at 39°C.

Sonstige Hinweise

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