293 N3S

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human kidney
• Verpackung: tube of 5 μg 92052131-DNA-5UG; pkg of vial of cells 92052131-1VL
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Adenovirus 5 early region (E1) antigens
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human embryo kidney

Beschreibung der Zelllinie

Adapted for growth in suspension by passaging 293 cells in nude mice. The cells express adenovirus 5 early region 1 (E1) antigens and are permissive for adenovirus 5 host range mutants defective in E1 functions.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 12,14
D16S539: 9
D5S818: 9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19

Nährmedium

Joklik′s MEM + 2mM Glutamine + 10% Horse Serum.

Subkultur-Routine

Maintain cultures between 5 x 10,000 to 5 x 100,000 cells/ml in a Techne stirrer; 5% CO₂; 37°C. Cell doubling time is 30 hours.

Sonstige Hinweise

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