UM-UC-13

8090508, human bladder, Epithelial

• Synonym(e): Human UM-UC-13, UM-UC-13 Cell Line
• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: UM-UC-13, 08090508
• Biologische Quelle: human bladder
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): metastasis
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human transitional cell carcinoma lymphatic metastasis

Beschreibung der Zelllinie

UM-UC-13 was produced from a lymphatic metastasis from a transitional cell carcinoma of the bladder. UM-UC-13 produced tumours in female athymic nude mice after 36 days. Susceptibility to adenoviral mediated gene transduction and expression of p53, p16, retinoblastoma protein and coxsackie adenovirus receptor were studied.

DNA-Profil

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8,12
D16S539: 11
D5S818: 9,13
D7S820: 8,9
THO1: 9
TPOX: 8
vWA: 16,18

Nährmedium

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37 °C.

Sonstige Hinweise

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Haftungsausschluss

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.