The activity and protein concentration of this product varies from lot to lot and are reported in the product Certificate of Analysis. This information may then be used to determine the lot specific volume. As an example, a lot reporting a 99 mg/mL protein concentration would result in a volume of 2.53 mL for the 250 mg package size. This 250 mg package would have a total activity of 2436 units. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/76427#product-documentation
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76427
Penicillin-Amidase aus E. coli
5-10 units/mg protein
Synonym(e):
Penicillin-Acylase
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250 MG
CHF 135.00
1 G
CHF 427.00
CHF 135.00
Voraussichtliches Versanddatum01. Juni 2025
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250 MG
CHF 135.00
1 G
CHF 427.00
About This Item
CHF 135.00
Voraussichtliches Versanddatum01. Juni 2025
Empfohlene Produkte
Biologische Quelle
Escherichia coli
Form
suspension
Spezifische Aktivität
5-10 units/mg protein
Mol-Gew.
Mr ~70000
Methode(n)
activity assay: suitable
Anwendung(en)
diagnostic assay manufacturing
Lagertemp.
2-8°C
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Allgemeine Beschreibung
Penicillin amidase is a periplasmic 80K heterodimer with A and B chains (209 and 566 amino acids, respectively). It is widely distributed among microorganisms, including bacteria, yeast and filamentous fungi. Among all the sources, the enzyme produced by E. coli is most well-characterized and common for industrial application.[1]
Anwendung
Penicillin amidase was used to study its effect in release of fatty acid and HSL (homoserine lactone) from AHLs (N -acylhomoserine lactones) in degradation of antibiotics.[2] It was used as positive control for assaying penicillin G acylase activity in the study of functional analysis of bile salt hydrolase and penicillin acylase family members in Lactobacillus sp.[3] Penicillin amidase may be used for synthesis of 6-aminopenicillanic acid from penicillin-G and for the industrial production of β-lactam antibiotics.[4]
Biochem./physiol. Wirkung
The biosynthesis of Penicillin amidase in E. coli by hydrophobic protein chromatography is an inducible reaction which is regulated by metabolized carbon source (e.g. polyols, carboxylic acid etc.). It is also influenced by catabolite repression.[5] It catalyzes the formation of amide bonds through an acyl-enzyme intermediate.[6]
Einheitendefinition
1 U entspricht der Enzymmenge, die 1 μmol Benzylpenicillin pro Minute bei pH 7.6 und 37°C hydrolysiert
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Penicillin Acylase in the Industrial Production of ?-Lactam Antibiotics
Bruggink A, Roos EC, Vroom ED
Organic Process Research & Development, 2(2), 128-133 (1998)
Lavinia Dunsmore et al.
Nature chemistry, 14(7), 754-765 (2022-06-29)
Natural products that contain ortho-quinones show great potential as anticancer agents but have been largely discarded from clinical development because their redox-cycling behaviour results in general systemic toxicity. Here we report conjugation of ortho-quinones to a carrier, which simultaneously masks
A. Guy et al.
Bioorganic & Medicinal Chemistry Letters, 3, 1041-1041 (1993)
Penicillin acylase (bacterial).
T A Savidge et al.
Methods in enzymology, 43, 705-721 (1975-01-01)
V Kasche et al.
Hoppe-Seyler's Zeitschrift fur physiologische Chemie, 365(12), 1435-1443 (1984-12-01)
Hydrophobic protein chromatography was used to prepare homogeneous fractions of penicillin amidase (EC 3.5.1.11) from E. coli. The apparent ratios of the rate constants for the deacylation of the acyl-penicillin amidase formed in the hydrolysis of phenylacetylglycine or D-phenylglycine methyl
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Good afternoon, I recently received Penicillin-Amidase aus E. coli 5-10 units/mg protein (250mg). Could you please guide me how much volume does it contain so that we can calculate the precise amount of concenteration available. Thank you in advance.
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