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Merck

57180

Sigma-Aldrich

Indo 1

for fluorescence, ≥80.0% (HPLC)

Synonym(e):

4-(6-Carboxy-2-indolyl)-4′-methyl-2,2′-(ethylendioxy)-dianilin-N,N,N′,N′-tetraessigsäure Pentakaliumsalz

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About This Item

Empirische Formel (Hill-System):
C32H26K5N3O12
CAS-Nummer:
Molekulargewicht:
840.05
MDL-Nummer:
UNSPSC-Code:
12352200
PubChem Substanz-ID:
NACRES:
NA.32

Qualität

for fluorescence

Assay

≥80.0% (HPLC)

Form

solid

Fluoreszenz

λex 331 nm; λem 398 nm in 0.1 M Tris pH 8.0, 10 mM Ca2+
λex 349 nm; λem 482 nm (100mM KCI)

Eignung

in accordance for fluorescence

Lagertemp.

−20°C

SMILES String

[K+].[K+].[K+].[K+].[K+].Cc1ccc(N(CC([O-])=O)CC([O-])=O)c(OCCOc2cc(ccc2N(CC([O-])=O)CC([O-])=O)-c3cc4ccc(cc4[nH]3)C([O-])=O)c1

InChI

1S/C32H31N3O12.5K/c1-18-2-6-24(34(14-28(36)37)15-29(38)39)26(10-18)46-8-9-47-27-13-20(5-7-25(27)35(16-30(40)41)17-31(42)43)22-11-19-3-4-21(32(44)45)12-23(19)33-22;;;;;/h2-7,10-13,33H,8-9,14-17H2,1H3,(H,36,37)(H,38,39)(H,40,41)(H,42,43)(H,44,45);;;;;/q;5*+1/p-5

InChIKey

VGZPRMSXVNDMAN-UHFFFAOYSA-I

Anwendung

Indo 1, a fluorescent calcium chelator and indicator, is used to monitor cytoplasmic Ca2+ transients induced by various physiological and mechanical processes.

Sonstige Hinweise

Fluorescent calcium chelator and indicator with an unusually large shift in fluorescence emission from 480 nm to 400 nm on binding of calcium. Useful for intracellular application

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

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