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IGEPAL® CA-630

Synonym(e):

′Nonidet™ P 40′, Nonylphenyl-polyethylenglykol, Octylphenoxy poly(ethyleneoxy)ethanol, branched, Octylphenoxy-Poly(ethylenoxy)ethanol, verzweigt

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50 ML
CHF 75.20
250 ML
CHF 194.00

CHF 75.20

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50 ML
CHF 75.20
250 ML
CHF 194.00

About This Item

Lineare Formel:
(C2H4O)nC14H22O
CAS-Nummer:
9002-93-1
MDL-Nummer:
MFCD00214058
UNSPSC-Code:
12161902
NACRES:
NA.25

CHF 75.20

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Beschreibung

non-ionic

Qualitätsniveau

100

Form

liquid

Mol-Gew.

~603.0 g/mol

Brechungsindex

n20/D 1.492

CMC

0.08 mM (20-25°C)

Übergangstemp.

cloud point 63-67  °C

Dichte

1.06 g/mL at 20 °C

Anionenspuren

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤100 mg/kg

Kationenspuren

Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤1000 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤1000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

HLB

13

SMILES String

O(CCO)c1ccc(cc1)C(CC(C)(C)C)(C)C

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChIKey

JYCQQPHGFMYQCF-UHFFFAOYSA-N

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Allgemeine Beschreibung

IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes. It is chemically indistinguishable from Nonidet P-40.[1]

Anwendung

IGEPAL® CA-630 has been used:
  • In phosducin co-precipitation assay for pull down of Epitope-Tagged HAGγ1 and HAGγ1C71S[2]
  • As a component of lysis buffer for cell culture and sample preparation for MAPK (mitogen-activated protein kinases) analysis, Immunoblotting[3][4]
  • In the extraction of cells in Oil Red O staining procedure to visualise lipid formation in cells during adipogenesis[4]
  • As a component of lysis buffer containing Tris-HCl, NaCl and EDTA for immunoblotting experiment[5]

Sonstige Hinweise

Nichtionisches, nicht-denaturierendens Detergens

Qualität

Chemisch nicht unterscheidbar von Nonidet P-40, das nicht mehr im Handel erhältlich ist.

Vorsicht

Kann sich während der Lagerung eintrüben oder absetzen. Durch Erhitzen auf 40 °C erhält man eine klare Flüssigkeit.
kann beim Lagern trüb werden; durch Erwärmen auf 40°C kann wieder ein klares Produkt erhalten werden

Rechtliche Hinweise

IGEPAL is a registered trademark of Solvay
Nonidet is a trademark of Shell Chemical Co.

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

483.8 °F - closed cup

Flammpunkt (°C)

251 °C - closed cup

Persönliche Schutzausrüstung

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
BCCM5020
BCCK6811
BCCH9235
BCCG8317
BCCF6744

Die passende Version wird nicht angezeigt?

Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.

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BCECF/AM A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF.

Sigma-Aldrich

216254

BCECF/AM

Farnesylation of the Transducin G Protein Gamma Subunit Is a Prerequisite for Its Ciliary Targeting in Rod Photoreceptors.
Frontiers in Molecular Neuroscience, 11, 16-16 (2018)
MEK?ERK and heparin-susceptible signaling pathways are involved in cell-cycle entry of the wound edge retinal pigment epithelium cells in the adult newt.
Yoshikawa T, et al.
Pigment Cell & Melanoma Research, 25(1), 66-82 (2012)
Selective mutations in estrogen receptor alpha' s D-domain alters nuclear translocation and non-ERE gene regulatory mechanisms.
Burns K A, et al.
The Journal of Biological Chemistry, jbc-M110 (2011)
Regulation of ERRa gene expression by estrogen receptor agonists and antagonists in SKBR3 breast cancer cells: differential molecular mechanisms mediated by G protein-coupled receptor GPR30/GPER-1.
Li Y, et al.
Molecular Endocrinology, 24(5), 969-980 (2010)
Arun K Mishra et al.
Molecular microbiology, 80(5), 1241-1259 (2011-03-26)
Lipomannan (LM) and lipoarabinomannan (LAM) are key Corynebacterineae glycoconjugates that are integral components of the mycobacterial cell wall, and are potent immunomodulators during infection. LAM is a complex heteropolysaccharide synthesized by an array of essential glycosyltransferase family C (GT-C) members
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Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. what is the concentration of this IGEPAL® CA-630 solution ?

    1 answer

    1. This product is considered a neat chemical, so you may assume 100% in your calculations.

      Helpful?

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