Direkt zum Inhalt
Merck
Alle Fotos(1)

Dokumente

60983

Millipore

Kovac-Reagenz für Indole

suitable for microbiology

Synonym(e):

4-Dimethylamino-benzaldehyd -Lösung, Indol-Reagens nach Kovac

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

Empirische Formel (Hill-System):
C9H11NO
CAS-Nummer:
100-10-7
Molekulargewicht:
149.19
Beilstein:
4132845
MDL-Nummer:
MFCD00003381
UNSPSC-Code:
41171621
PubChem Substanz-ID:
329759335
NACRES:
NA.85

Haltbarkeit

limited shelf life, expiry date on the label

Qualitätsniveau

100

Zusammensetzung

n-butanol
4-dimethylaminobenzaldehyde
hydrochloric acid

Methode(n)

microbe id | specific enzyme detection: suitable

Brechungsindex

n20/D 1.400-1.420

Anwendung(en)

agriculture
clinical testing
environmental
food and beverages
microbiology

Lagertemp.

2-8°C

Eignung

Escherichia coli
coliforms

SMILES String

[H]C(=O)c1ccc(cc1)N(C)C

InChI

1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3

InChIKey

BGNGWHSBYQYVRX-UHFFFAOYSA-N

Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich

Verwandte Kategorien

Allgemeine Beschreibung

Kovac′s reagent for indoles is a solution of 4-(dimethylamino)benzaldehyde and hydrochloric acid in n-butanol.

Anwendung

Kovac′s reagent for indoles has been used for enumerating E. coli O157:H7 in ground beef samples in different scenarios of stored, thawed, and as cooked patty samples. It has also been used for E coli enumeration in dewatered blackwater.

Biochem./physiol. Wirkung

Kovac′s reagent detects the indole and aids in identifying the indole-positive and indole-negative microorganisms. E. coli can split tryptophan into indole and α-aminopropionic acid in the presence of the enzyme tryptophanase. p-Aminobenzaldehyde present in the reagent reacts with indole to form a cherry-red complex, that is soluble in alcohol, ether, and chloroform. It is recommended to use glucose free media and peptone with a high tryptophan content for the test.

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Central nervous system, Respiratory system

Lagerklassenschlüssel

3 - Flammable liquids

WGK

WGK 1

Flammpunkt (°F)

96.8 °F

Flammpunkt (°C)

36 °C

Persönliche Schutzausrüstung

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Choose from one of the most recent versions:

Analysenzertifikate (COA)

Lot/Batch Number
BCCL0925
BCCK8015
BCCM2245
BCCJ6939
BCCG3903

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

G L Lombard et al.
Journal of clinical microbiology, 18(3), 609-613 (1983-09-01)
Three reagents for detecting indole, Kovac, Ehrlich, and p-dimethylaminocinnamaldehyde (DMCA), were evaluated with commercial microtest systems for characterizing and identifying anaerobic bacteria. The DMCA reagent, the most sensitive of the three reagents, gave a positive reaction with 445 of 449
Ragnhildur Gunnarsdóttir et al.
Environmental science & technology, 46(22), 12408-12416 (2012-11-02)
Wastewater treatment in many Arctic regions is inadequate, even nonexisting. Natural freezing of wastewater in those areas may be beneficial for reduction of microorganisms. The aim of this study was to investigate the effect of long-term freezing, and repeated freezing
Stavros G Manios et al.
Meat science, 101, 25-32 (2014-12-03)
The effect of common handling practices (i.e., freezing, thawing and cooking) of beef patties on the survival of Salmonella spp. and Escherichia coli O157:H7, was evaluated. Inoculated ground beef was stored at -22 °C for 5 and 75 days. After
M Stefek et al.
Biochimica et biophysica acta, 1502(3), 398-404 (2000-11-09)
In the present work, pepsin digests of tail tendons from streptozotocin-diabetic rats were found to contain material that reacted rapidly at room temperature with p-dimethylaminobenzaldehyde (Ehrlich's reagent) to give an adduct with an absorbance spectrum characteristic of the Ehrlich chromogen
Rosario Zamora et al.
Journal of agricultural and food chemistry, 52(13), 4166-4171 (2004-06-24)
The Ehrlich reaction was optimized to determine the formation of pyrrolized phospholipids in edible oils in an attempt to understand the color reversion produced during the deodorization of poorly degummed edible oils. The procedure consisted of the treatment of the
Alle zugehörigen Dokumente anzeigen

Artikel

Differentiation of Escherichia coli from coliforms

There are many other methods of detection to indicate the presence of E. coli. Review common tests and biochemical reactions for this contaminant.

Identification of Microorganisms Based on Color

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram.

Detection, Identification and Differentiation of Clostridium perfringens

Clostridium perfringens can cause contamination in undercooked or improperly sterilized canned foods and water. Learn to detect, identify, and differentiate this pathogen.

Pseudomonas Media and Tests

An article concerning the detection, identification, differentiation, and cultivation of Pseudomonas species.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.