MAB10764
Anti-SBP-tag Antibody, clone 20 | MAB10764
clone 20, 1.0 mg/mL, from mouse
Synonym(e):
streptavidin binding peptide
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About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
purified immunoglobulin
Antikörper-Produkttyp
primary antibodies
Klon
20, monoclonal
Speziesreaktivität
human
Konzentration
1.0 mg/mL
Methode(n)
immunocytochemistry: suitable
western blot: suitable
Isotyp
IgG1κ
Versandbedingung
wet ice
Posttranslationale Modifikation Target
unmodified
Allgemeine Beschreibung
Streptavidin Binding Peptide tag is a useful and versatile peptide that can be inserted into expression vectors for easy detection of recombinant proteins and purification. The sequence for SBP-tag is 38 amino acids long having a nanomolar scale binding affinity for streptavidin with an equilibrium constant of 2.5 nM.
Spezifität
Demonstrated to react with cells expressing the SBP tag.
This antibody recognizes SBP tag.
Immunogen
Epitope: Unknown
Linear peptide corresponding to SBP tag.
Anwendung
Research Category
Epitop-Tags & allgemeine Anwendung
Epitop-Tags & allgemeine Anwendung
Research Sub Category
Epitop-Tags
Epitop-Tags
Immunofluorescence Analysis: A previous lot was used by an independent laboratory in IF. (Hudson, D., et al. (2008). MBC. 19(7):3070-3079.)
Qualität
Evaluated by Western Blot in pTrAP-GFP transfected Hek293 lysate.
Western Blot Analysis: 0.1 ng/mL of this antibody detected SBP-tag on 10 µg of pTrAP-GFP transfected Hek293 lysate.
Western Blot Analysis: 0.1 ng/mL of this antibody detected SBP-tag on 10 µg of pTrAP-GFP transfected Hek293 lysate.
Zielbeschreibung
The protein expresss is pTrAP-GFP plasmids containing a Histidine tag, S-tag, streptavidin-binding pepide (SBP) tag, and GFP. The protein expressing is ~37 kDa
Physikalische Form
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide
Lagerung und Haltbarkeit
Stable for 1 year at 2-8°C from date of receipt.
Hinweis zur Analyse
Control
pTrAP-GFP transfected Hek293 lysate
pTrAP-GFP transfected Hek293 lysate
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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