CP28
Anti-Fibroblast Antigen Mouse mAb (AS02)
liquid, clone AS02, Calbiochem®
Synonym(e):
Anti-CD90 (Ab-1) (Mouse), Anti-Thy-1 (Ab-1) (Mouse)
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About This Item
UNSPSC-Code:
12352203
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
purified antibody
Antikörper-Produkttyp
primary antibodies
Klon
AS02, monoclonal
Form
liquid
Enthält
≤0.1% sodium azide as preservative
Speziesreaktivität
human, monkey
Darf nicht reagieren mit
rat, mouse
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
OK to freeze
Isotyp
IgG1
Versandbedingung
wet ice
Lagertemp.
2-8°C
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... FGF22(27006)
Allgemeine Beschreibung
Anti-Fibroblast Antigen, mouse monoclonal, clone AS02, detects ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts (non-reducing conditions). It is validated for FC, WB, IF, IP & IHC (frozen sections).
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with X63-Ag.8.653 mouse myeloma cells. Recognizes the ~30-31 kDa fibroblast antigen (CD90/Thy-1) under non-reducing conditions.
Recognizes the ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts under non-reducing conditions.
Immunogen
Human
human fibroblasts
Anwendung
Flow Cytometry (see comments)
Frozen Sections (10 µg/ml, acetone fixation)
Immunoblotting (10 µg/ml, non-reducing only)
Immunofluorescence (10 µg/ml)
Immunoprecipitation (2-10 µg/ml)
Frozen Sections (10 µg/ml, acetone fixation)
Immunoblotting (10 µg/ml, non-reducing only)
Immunofluorescence (10 µg/ml)
Immunoprecipitation (2-10 µg/ml)
Verpackung
Please refer to vial label for lot-specific concentration.
Warnhinweis
Toxicity: Standard Handling (A)
Physikalische Form
In 50 mM sodium phosphate buffer, 6% BSA.
Hinweis zur Analyse
Positive Control
Any fibroblast cell line or skin tissue
Any fibroblast cell line or skin tissue
Sonstige Hinweise
Saalbach, A., et al. 1996 J. Invest. Dermatol.106, 1314.
Janin, A., et al. 1990. Clin. Exp. Rheum.8, 237.
Kay, M., et al. 1989. J. Invest. Dermatol.92, 168.
Esterre, P.H., et al. 1984 Cell Mol. Biol.38, 297.
Hoyhtya, M., et al. 1984 Eur. J. Biochem141, 477.
Janin, A., et al. 1990. Clin. Exp. Rheum.8, 237.
Kay, M., et al. 1989. J. Invest. Dermatol.92, 168.
Esterre, P.H., et al. 1984 Cell Mol. Biol.38, 297.
Hoyhtya, M., et al. 1984 Eur. J. Biochem141, 477.
This antibody has been used with magnetic beads to remove fibroblasts from mixed cultures. It is also suitable for flow cytometry studies (see application references). Note that in healthy tissue (skin, placenta, liver, heart), this antibody does not react with endothelial cells. However, endothelial cells activated by PMA, TNFα, or IFNγ may be detected by this antibody, suggesting activated endothelial cells may express CD90 and that CD90 may be an activation marker of human endothelial cells. Antibody should be titrated for optimal results in individual systems.
Rechtliche Hinweise
Manufactured by Dianova, GmbH, Germany.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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