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AB5589

Sigma-Aldrich

Anti-Potassium Channel Kv1.3 Antibody, extracellular domain

Chemicon®, from rabbit

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Antikörperform

affinity purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Aufgereinigt durch

affinity chromatography

Speziesreaktivität

rat, human

Hersteller/Markenname

Chemicon®

Methode(n)

flow cytometry: suitable
western blot: suitable

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... KCNA3(3738)

Spezifität

Recognizes Kv1.3, extracellular (Kcna3).

Immunogen

Epitope: extracellular domain
Highly purified peptide corresponding to residues 263-275 of human Kv1.3 (Accession P22001).

Anwendung

Research Category
Neurowissenschaft
Research Sub Category
Ionenkanäle & -transporter
This Anti-Potassium Channel Kv1.3 Antibody, extracellular domain is validated for use in FC, WB for the detection of Potassium Channel Kv1.3.
Western blotting: 1:200-1:500 using ECL on rat brain membranes.

Indirect flow cytometry: 0.5-5 μg of antibody per 1x10e6cells.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Physikalische Form

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Lagerung und Haltbarkeit

Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain antibody and control peptide at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Hinweis zur Analyse

Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). For negative control, preincubate 5 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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H-Sätze

P-Sätze

Gefahreneinstufungen

Aquatic Chronic 3

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Luiz O Leiria et al.
The Journal of physiology, 591(9), 2259-2273 (2013-03-13)
We aimed to investigate the role of insulin in the bladder and its relevance for the development of overactive bladder (OAB) in insulin-resistant obese mice. Bladders from male individuals who were involved in multiple organ donations were used. C57BL6/J mice
Ameet A Chimote et al.
The Journal of biological chemistry, 287(3), 2055-2067 (2011-12-03)
Hypoxia in solid tumors contributes to decreased immunosurveillance via down-regulation of Kv1.3 channels in T lymphocytes and associated T cell function inhibition. However, the mechanisms responsible for Kv1.3 down-regulation are not understood. We hypothesized that chronic hypoxia reduces Kv1.3 surface
Zerrin Kuras et al.
PloS one, 7(8), e43859-e43859 (2012-09-07)
The migration of T lymphocytes is an essential part of the adaptive immune response as T cells circulate around the body to carry out immune surveillance. During the migration process T cells polarize, forming a leading edge at the cell

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