AB3150
Anti-Fractin Antibody, CT
serum, Chemicon®
Synonym(e):
32 kDa Fragment of beta-Actin
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)
About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
rabbit
Qualitätsniveau
Antikörperform
serum
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Speziesreaktivität
human, rat, mouse
Hersteller/Markenname
Chemicon®
Methode(n)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Posttranslationale Modifikation Target
unmodified
Allgemeine Beschreibung
During apoptosis, beta actin is cleaved by activated caspase 3 between Asp244 and Gly245. This cleavage results in the release of an approximately 130 amino acid (15 kDa) fragment from the C-terminus of beta actin and a 32 kDa (244 amino acid) N-terminal fragment of actin, known as Fractin. The C-terminus of the Fractin fragment represents an apoptosis-specific neoepitope that is not antigenic in native full-length beta actin and therefore presents a novel and specific marker for distinguishing apoptotic from necrotic cells.
Spezifität
By immunohistochemistry and Western blot, this antibody only recognizes the 32 kDa Fractin fragment but not full-length beta actin.
Immunogen
K-YELPD peptide representing the last five amino acids of the C-terminus of the 32 kDa Fractin fragment of beta-Actin. This sequence is absolutely conserved in Fractin fragments from human, mouse, rat, feline, porcine, ovine, bovine, canine, rabbit and guinea pig origin.
Anwendung
Detect Fractin using this Anti-Fractin Antibody, C-terminus validated for use in WB, IC, IH(P).
Immunohistochemistry/Immunocytochemistry: 1:400-1:1000. 1 hr at 37°C or 4°C overnight. Note: If studying paraffin-embedded tissue, the use of antigen retrieval methods is recommended.
Western Blot: 1:1000-1:4000 for incubation overnight at 4°C. Recommended antibody dilution buffer: TBS with 3% BSA and 0.05% Tween 20, pH 7.4
Optimal working dilutions must be determined by end user.
Western Blot: 1:1000-1:4000 for incubation overnight at 4°C. Recommended antibody dilution buffer: TBS with 3% BSA and 0.05% Tween 20, pH 7.4
Optimal working dilutions must be determined by end user.
Physikalische Form
Liquid whole rabbit serum containing no preservatives.
Lagerung und Haltbarkeit
Aliquot and store at -20°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles.
Hinweis zur Analyse
Control
POSITIVE CONTROL: Staurosporin treated neuronal cultures.
POSITIVE CONTROL: Staurosporin treated neuronal cultures.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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Zhouheng Ye et al.
Journal of neuropathology and experimental neurology, 77(3), 260-265 (2018-02-07)
Our previous study demonstrated caspase independent DNA fragmentation after very brief cerebral ischemia, the mechanism of which was unclear. In this study, we explore whether actin is cleaved following focal cerebral ischemia, and whether these structural changes of actin might
Peter L Peng et al.
Neuron, 49(5), 719-733 (2006-03-01)
ADAR2 is a nuclear enzyme essential for GluR2 pre-mRNA editing at Q/R site-607, which gates Ca2+ entry through AMPA receptor channels. Here, we show that forebrain ischemia in adult rats selectively reduces expression of ADAR2 enzyme and, hence, disrupts RNA
Vanessa Ginet et al.
BioFactors (Oxford, England), 42(3), 323-336 (2016-06-18)
Lactoferrin (Lf), component of maternal milk, has antioxidant, anti-inflammatory and antimicrobial properties. Neuroprotective effects of Lf on the immature brain have been recently shown in rodent models of intrauterine growth restriction and cerebral hypoxia/ischemia. Here we postulated that Lf could
Yohan van de Looij et al.
Annals of clinical and translational neurology, 1(12), 955-967 (2015-01-13)
Lactoferrin (Lf) is an iron-binding glycoprotein secreted in maternal milk presenting anti-inflammatory and antioxidant properties. It shows efficient absorption into the brain from nutritional source. Brain injury frequently resulting from cerebral hypoxia-ischemia (HI) has a high incidence in premature infants
Alcohol-induced apoptosis of oligodendrocytes in the fetal macaque brain.
Creeley, CE; Dikranian, KT; Johnson, SA; Farber, NB; Olney, JW
Acta Neuropathologica Communications null
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