Reacts with purified elementary bodies, disrupted. The unconjugated antibody reacts at >1:1,000 by indirect immunofluorescence vs. all serovars (A-K, L1-L3). Cross-reacts with Chlamydia psittacii and Chlamydia pneumoniae (TWAR). Non reactive with HEp-2 cells and egg yolk sac.
Immunogen
L2 and other serovar groups
Anwendung
Applications include direct FA staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining.
Titer by indirect immunofluorescence: >1:1,000 vs. EB′s (all serovars, A-K, L1-L3).
Working dilutions must be determined by end user (suggested starting range 1:10-1:50).
Detect Chlamydia trachomatis-EB using this Anti-Chlamydia trachomatis-EB Antibody, FITC-conjugated validated for use in IF.
Physikalische Form
IgG fraction conjugated with FITC. Supplied in PBS (0.1 M, pH 7.2) with 10 mg/mL BSA as a stabilizer and 0.1% sodium azide.
Lagerung und Haltbarkeit
Maintain at -20°C in undiluted aliquots for up to 12 months or at 2-8°C for up to 3 months after date of receipt. Avoid repeated freeze/thaw cycles. Store under subdued light.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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The invasion of Chlamydia trachomatis, an obligate intracellular bacterium, into epithelial cells is driven by a complex interplay of host and bacterial factors. To comprehensively define the host genes required for pathogen invasion, we undertook a fluorescence-activated cell sorting (FACS)-based
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