5.00526

NOX Inhibitor VII, Thr101

• Synonym(e): NOX Inhibitor VII, Thr101, NADPH Oxidase Inhibitor VII, 6-Fluoro-2- o-tolylbenzo[d]isothiazol-3(2H)-one, 6-Fluoro-2-(2-methylphenyl)-1,2-benzothiazol-3(2H)-one
• Empirische Formel (Hill-System): C₁₄H₁₀FNOS
• CAS-Nummer: 727664-79-1
• Molekulargewicht: 259.30
• UNSPSC-Code: 12352200
• NACRES: NA.77

Eigenschaften

• Assay: ≥98% (HPLC)
• Qualitätsniveau: 100
• Form: powder
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; protect from light
• Farbe: light brown
• Löslichkeit: DMSO: 50 mg/mL
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

A cell-permeable, selenium-to-sulfur substituted Ebselen (Cat. No. 324483) derivative that exhibits similar NOX2 inhibitory activity as Ebselen by blocking p22phox c-terminal PRD (proline-rich domain) and p47phoxbis-SH3 interaction. Although less potent than Ebselen in cell-free p22phox PRD-p47phoxbis-SH3 binding (IC₅₀ = 4 vs. 0.3 µM) and NOX2 activity (IC₅₀ = 4 vs. 0.6 µM) assays, Thr101 is more potent and NOX2-selective than Ebselen in cell-based assays (Thr101 IC₅₀ /Ebselen IC₅₀ = 0.3/0.5, 3/0.15, 8/no inhibition, 8/0.7 µM, respectively, against NOX2, NOX1, NOX4, and NOX5) and exhibits no glutathione peroxidase activity.

A cell-permeable, selenium-to-sulfur substituted Ebselen (Cat. No. 324483) derivative that exhibits similar NOX2 inhibitory activity as Ebselen by blocking p22phox c-terminal PRD (proline-rich domain) and p47phoxbis-SH3 interaction. Although less potent than Ebselen in cell-free p22phox PRD-p47phoxbis-SH3 binding (IC₅₀ = 4 vs. 0.3 µM) and NOX2 activity (IC₅₀ = 4 vs. 0.6 µM) assays, Thr101 is more potent and NOX2-selective than Ebselen in cell-based assays (Thr101 IC₅₀ /Ebselen IC₅₀ = 0.3/0.5, 3/0.15, 8/no inhibition, 8/0.7 µM, respectively, against NOX2, NOX1, NOX4, and NOX5) and exhibits no glutathione peroxidase activity.

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

Biochem./physiol. Wirkung

Cell permeable: yes

Primary Target
blocks p22phox c-terminal PRD (proline-rich domain) and p47phoxbis-SH3 interaction

Verpackung

Packaged under inert gas

Warnhinweis

Toxicity: Standard Handling (A)

Rekonstituierung

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sonstige Hinweise

Smith, S.M.E., et al. 2012. Chem. Biol.19, 752.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable