07-502-I

Anti-p67-phox (not Human) Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Neutrophil cytosol factor 2, NCF-2, 67 kDa neutrophil oxidase factor, NADPH oxidase activator 2, Neutrophil NADPH oxidase factor 2, p67-phox, p67-phox (rodent)
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: rat, mouse
• Methode(n): immunohistochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_035007
• UniProt-Hinterlegungsnummer: O70145
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Ncf2(17970) ; rat ... Ncf2(364018)

Beschreibung

Allgemeine Beschreibung

Neutrophil cytosol factor 2 (UniProt Q09014; also known as 67 kDa neutrophil oxidase factor, NADPH oxidase activator 2, NADPH oxidase subunit 67 kDa, NCF-2, Ncf2, Neutrophil NADPH oxidase factor 2, p67-phox, p47phox) is encoded by the murine Ncf2 (also known as Noxa2, p67phox) gene in murine species (Gene ID 17970). Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors, including p47-phox, p67-phox, p40-phox and Rac. In the resting cell p47phox, p67phox, and p40phox form a large molecular weight cytosolic complex. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of mouse p67-phox.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Entwicklungsabhängige Signalübertragung

Anti-p67-phox (not Human) Antibody is an antibody against p67-phox, tested in mouse and rat, but is not expected to react with human, for use in Western Blotting and Immunohistochemistry.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected p67-phox (rodent) in rat colon and mouse hindbrain tissue.

Qualität

Evaluated by Western Blotting in C6 and THP-1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected p67-phox (rodent) in 10 µg of C6 and THP-1 cell lysate.

Zielbeschreibung

~72 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physikalische Form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable