X100RS

Triton^™ X-100 reduced

• Synonyme(s) : Polyoxyethylene (10) isooctylcyclohexyl ether
• Formule linéaire : 4-(C₈H₁₇)C₆H₁₀(OCH₂CH₂)_nOH, n~10
• Numéro CAS: 92046-34-9
• Numéro MDL: MFCD00167048
• Code UNSPSC : 12161900
• Nomenclature NACRES : NA.25

Propriétés

• Source biologique: synthetic (oragnic)
• Niveau de qualité: 100
• Description: non-ionic
• Forme: viscous liquid
• Poids mol.: micellar average mol wt 80,000; average mol wt 625
• Nombre d'agrégation: 100-155
• Indice de réfraction: n20/D 1.473 (lit.)
• CMC: 0.2-0.9
• Pf: 48 -50 °C (118 - 122 °F)
• Température de transition: cloud point 65 °C
• Solubilité: water: soluble, clear to slightly hazy, colorless
• Densité: 1.029 g/mL at 25 °C (lit.)
• Absorption: ≤0.25 at 277 nm in H₂O at 0.5%
• HLB: 13.5
• Chaîne SMILES: CC(C)(C)CC(C)(C)C1CCC(CC1)OCCOCCOCCOCCOCCOCCOCCO
• InChI: 1S/C28H56O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h25-26,29H,6-24H2,1-5H3
• Clé InChI: QQJNBKDKLMCALZ-UHFFFAOYSA-N

Description

Description générale

Triton^™ X-100 reduced is a versatile nonionic surfactant featuring a hydrophilic polyethylene oxide chain, commonly employed as a laboratory detergent. Its applications span various purposes, including cell lysis to extract proteins or organelles, permeabilizing the membranes of living cells, and serving as a vital component in lysis buffers. Widely utilized in biochemical and cell biology research, Triton^™ X-100 facilitates the isolation of lipid rafts, enhancing the solubility and dispersibility of substances. With a reduced polyoxyethylene content of approximately 10, this product exhibits excellent wetting properties and emulsification enhancement.

In cell biology, Triton^™ X-100 plays a crucial role in solubilizing membrane-bound proteins, preserving their native conformation in solution. Furthermore, Triton^™ X-100 reduced, resulting from the full hydrogenation of the benzene moiety of TX-100 to a cyclohexane derivative, has been associated with potential benefits in enzyme digestion enhancement and effects on the photoisomerization of bacteriorhodopsin. The versatility of Triton^™ X-100 in various research applications underscores its significance as a fundamental tool in biochemical and cell biology investigations.

Application

Triton^™ X-100 reduced has been used:

• in nuclear magnetic resonance (NMR) sample tube for lysing/rupturing the 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) phospholipid vesicles
• in cell permeabilization
• as a component in Tris/HCl to determine hydrolysis of the esters for enzyme activity assays

Caractéristiques et avantages

• Non-ionic surfactant
• Improves solubility and dispersibility of substances
• Excellent wetting properties
• Enhances emulsification
• High purity product for research applications

Notes préparatoires

Hydrogenated to reduce UV absorbance.

Autres remarques

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Informations légales

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Informations sur la sécurité

• Pictogrammes: GHS07,GHS09
• Mention d'avertissement: Warning
• Mentions de danger: H315,H319,H335,H411
• Conseils de prudence: P261 - P264 - P271 - P273 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 235.4 °F - closed cup
• Point d'éclair (°C): 113 °C - closed cup
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves