SAE0159

Microbial Transglutaminase

lyophilized powder, ≥12 units/mg protein

• Synonyme(s) : Microbial Enzyme, Transglutaminase, Transglutaminase Enzyme
• NACRES: NA.54
• UNSPSC Code: 12352204
• Specific activity: ≥12 units/mg protein

Propriétés

• form: lyophilized powder
• Quality Segment: 200
• specific activity: ≥12 units/mg protein
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Transglutaminases (TG) are a family of enzymes that catalyze isopeptide bond formation. This bond formation occurs between the γ-carboxyamide group of glutamine and various primary amines (primarily the ε-amino group of lysine). The resulting intermolecular or intramolecular cross-linking of transglutaminase is highly stable and shows high resistance to proteolytic degradation. The transglutaminase crosslinking activity support the formation of blood clots , skin and hair. On the other hand, TG is now being implicated in Celiac disease as well as in Huntington and Parkinson diseases. Historically, Microbial transglutaminase has been heavily used in the food industry. Microbial TG is often preferred for newer applications (such as site-specific protein modification and antibody drug conjugation) due to its small molecular weight and lack of calcium dependency when compared to the mammalian forms.

Application

Microbial Transglutaminase has been used for:

• Protein cross-linking & site-specific labeling
• Antibody Drug Conjugation
• 3D bioprinting bioink preparation
• Food related immunogenicity/pathogenicity related research

Features and Benefits

• Small (~38kDa) and calcium independent enzyme
• Highly purified lyophilized enzyme
• Consistent and reproducible activity
• Characterized for endotoxin content

Preparation Note

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices

Store the freeze-dried product at –20 °C. It is recommended to store the reconstituted protein in working aliquots at –20 °C to avoid repeated freeze/ thaw cycles.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mot-clé: Danger
• Codes de danger: H334
• Mentions de précaution: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Classe de stockage: 11 - Combustible Solids
• Que se passe-t-il ?: WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable