Accéder au contenu
Merck
Toutes les photos(4)

Key Documents

S3062

Sigma-Aldrich

Anti-α-Synuclein antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonyme(s) :

Anti-SNCA

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen 19 kDa

Espèces réactives

rat, human

Technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formic acid treated sections of Alzheimer′s Disease brain
microarray: suitable
western blot: 1:1,000 using extract of rat brain homogenate

Numéro d'accès UniProt

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... SNCA(6622)
rat ... Snca(29219)

Description générale

The synucleins are a family of soluble presynaptic proteins that are abundant in neurons and include α-synuclein, β-synuclein, and γ-synuclein. Human α-synuclein (also known as the non-amyloid component of plaques precursor protein or NACP) is a 140-amino acid polypeptide that is encoded by a gene on chromosome 4. It was originally isolated from plaques of Alzheimer′s disease (AD) brains as a 19 kDa protein precursor of the highly hydrophobic 35-amino acid peptide, nonamyloid component (NAC) of plaques. The NAC can self-aggregate into fibrils and induce aggregation of the β-amyloid peptide. α-Synuclein is highly abundant in presynaptic terminals, and is a major component of Lewy bodies (LBs). Pathogenic point mutations in the α-synuclein gene are linked to familial Parkinson′s disease (PD) in rare kindreds. However, most neurodegenerative disorders with LBs are associated with abnormal accumulation of wild-type, α-synuclein. α-Synuclein is expressed primarily in brain, but is also expressed in low levels in all tissues examined except liver.

Spécificité

Rabbit polyclonal anti-α-Synuclein antibody recognizes human and rat α-synuclein. Staining of α-synuclein in immunoblotting is specifically inhibited by the immunizing peptide

Immunogène

synthetic peptide corresponding to a sequence near the C-terminus of human α-synuclein (amino acids 111-132 with C-terminally added lysine) conjugated to KLH. This sequence is highly conserved (90% homology) in mouse and rat α-synuclein. This sequence has no homology with β- and γ-synuclein.

Application

Rabbit polyclonal anti-α-Synuclein antibody is used to tag α-Synuclein for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques, such as immunoblotting. It is also used as a probe to determine the presence and roles of α-Synuclein in plaques of Alzheimer′s disease (AD) brains.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Identification of a caspase-derived N-terminal tau fragment in cellular and animal Alzheimer's disease models
Corsetti V, et al.
Molecular and Cellular Neurosciences, 38(3), 381-392 (2008)
Extracellular truncated tau causes early presynaptic dysfunction associated with Alzheimer?s disease and other tauopathies
Florenzano F, et al.
Oncotarget, 8(39), 64745-64778 (2017)
Clinical, Histological, and Immunohistochemical Findings in Inclusion Body Myositis
Camargo L, et al.
BioMed Research International, 2018 (2018)
Lysosomal dysfunction in the brain of a mouse model with intraneuronal accumulation of carboxyl terminal fragments of the amyloid precursor protein
Kaur G, et al.
Molecular Psychiatry, 22(7), 981-981 (2017)
Geert Callewaert et al.
Frontiers in genetics, 11, 266-266 (2020-05-28)
The yeast Saccharomyces cerevisiae is a powerful model to study the molecular mechanisms underlying α-synuclein (α-syn) cytotoxicity. This is due to the high degree of conservation of cellular processes with higher eukaryotes and the fact that yeast does not endogenously

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique