P9041
Phosphodiesterase II from bovine spleen
lyophilized powder, ≥5.0 units/mg protein
Synonyme(s) :
3′-Exonuclease, Spleen exonuclease, Spleen phosphodiesterase
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About This Item
Produits recommandés
Forme
lyophilized powder
Activité spécifique
≥5.0 units/mg protein
Poids mol.
65 kDa
Numéro d'accès UniProt
Activité étrangère
5′-Nucleotidase ≤1% of base activity
Température de stockage
−20°C
Informations sur le gène
cow ... PDE2A(281971)
Description générale
Research area: Cell signaling. Phosphodiesterase (PDE) II belongs to the PDE superfamily and includes subtypes from one to 11. They exist as a dimer and comprise of N-terminal regulatory domain, C-terminal prenylated domain, and a Zn2+containing catalytic domain. The bovine spleen PDE2 corresponds to a molecular weight of 65 kDa. It requires divalent cations for its enzymatic activity and has an optimum pH of 7.5.
Application
Phosphodiesterase (PDE) is any enzyme that is used to break phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase II has been used in the enzymatic digestions of purified proteins such as the P8-dGMP complex. Bovine spleen phosphodiesterase has been used to digest N-cadherin. Phosphodiesterase II from bovine is suitable for sequential and end-group analysis of poly and oligonucleotides. It may be used in kinetic studieson substrate degradation of macro-molecules.
Phosphodiesterase II from bovine spleen has been used in the:
- excision of pyridyloxobutyl (POB) base adducts from DNA
- digestion of DNA prior to cycloadenosine enrichment
- hydrolysis of DNA from blue mussels gill tissue into deoxyribonucleoside 3′-monophosphates
The product has been used in the characterization of polynucleotide chain length, base composition, and identity of terminal nucleotide. The enzyme has also been used in excision of pyridyloxobutyl (POB) base adducts from DNA. Furthermore, it has been used along with micrococcal endonuclease to hydrolyze purified DNA to 3 -nucleoside monophosphates.
Actions biochimiques/physiologiques
Phosphodiesterase (PDE) breaks phosphodiester bonds. 3-Isobutyl-methylxanthine (IBMX) is a potential PDE2 inhibitor.
The enzyme acts on poly(A), poly(U), and poly(I). Native DNA and poly(C) are quite resistant to the action of this enzyme.
Hydrolyzes RNA, RNA-Core, 3′-alkyl- and 3′-aryl-nucleoside phosphates, and polydeoxyribonucleotides with 3′-phosphate end groups to 3′-mononucleotides.
Polynucleotides having 5′-phosphomonoester end groups are not attacked.
Polynucleotides having 5′-phosphomonoester end groups are not attacked.
Définition de l'unité
One unit will produce acid soluble nucleotides equivalent to a ΔA260 of 16 in 30 min at pH 6.5 at 37 °C, in a 2.0 mL reaction mixture. Substrate: RNA-Core. Actual A260 is measured on the supernatant after precipitation of the unhydrolyzed RNA with uranyl acetate-perchloric acid reagent.
Remarque sur l'analyse
Protein determined by biuret
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificats d'analyse (COA)
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Les clients ont également consulté
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The Journal of organic chemistry, 70(25), 10453-10460 (2005-12-06)
[reaction: see text] An effective method for the synthesis of 2'-O-cyanoethylated oligoribonucleotides as a new class of 2'-O-modified RNAs was developed. The reaction of appropriately protected ribonucleoside derivatives with acrylonitrile in t-BuOH in the presence of Cs2CO3 gave 2'-O-cyanoethylated ribonucleoside
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Donald H Maurice
Proceedings of the Western Pharmacology Society, 46, 32-36 (2004-01-01)
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