MWGF1000
Gel Filtration Markers Kit for Protein Molecular Weights 29,000-700,000 Da
Synonyme(s) :
Gel Filtration Markers Kit, Gel Filtration Protein Ladder, Protein Molecular Weight Marker Kit
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.25
Produits recommandés
Forme
liquid
Niveau de qualité
Technique(s)
liquid chromatography (LC): suitable
Température de stockage
−20°C
Description générale
Gel filtration chromatography is a well-known technique for measuring the size and molecular weight of proteins. It separates proteins based on their ability to diffuse into the pores of the resin. Larger proteins have more difficulty entering the resin pores, so they flow through the column more quickly than smaller proteins. As a result, the proteins are eluted from the column in order of decreasing molecular weight.
Application
Gel Filtration Markers Kit for Protein Molecular Weights 29,000-700,000 Da has been used:
- for protein purification and analyses (gel-filtration experiments)
- for size-exclusion chromatography/gel filtration chromatography of synaptoneurosome extract
- for glycerol gradient centrifugation
- to study glucocorticoid-induced lymphocytolysis as a model system for apoptosis within the immune system
- to isolate Polygonatum odoratum lectin, which showed remarkable anti-HSV-II activity towards Vero cells, cytotoxicity towards human melanoma A375 cells and induced apoptosis in a caspase-dependent manner
Actions biochimiques/physiologiques
The method described in the bulletin for determining molecular masses using gel filtration chromatography is a modified version of existing published techniques. The protein standards included in this kit may be compatible with other chromatographic systems like high-performance liquid chromatography (HPLC). However, certain buffer systems may affect the elution volumes of albumin and carbonic anhydrase. The proteins in this kit have a molecular mass range spanning from 29 kDa to 699 kDa.
Autres remarques
- For research use only. Not for drug, household, or other uses.
- Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
- Please refer to the product information sheet to learn more about reagents, storage and stability, and procedure.
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
- A8531Albumin, bovine serum, 66,000 MW 1 mL/vialFDS
- A8656Alcohol Dehydrogenase, yeast, 150,000 MW 1 mL/vialFDS
- A8781 β-Amylase, sweet potato, 200,000 MW 1 mL/vialFDS
- A3660 Apoferritin, horse spleen, 443,000 MW 1 mL/vialFDS
- D4772Blue dextran, 2,000,000 MW 1 mL/vialFDS
- C7025Carbonic Anhydrase, bovine erythrocytes, 29,000 MW 1 mL/vialFDS
- T9145Thyroglobulin, bovine, 669,000 MW 1 mL/vialFDS
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Les clients ont également consulté
D K Wimsatt et al.
Clinical chemistry, 33(11), 2100-2106 (1987-11-01)
The Coomassie Brilliant Blue G-250 method for urinary proteins underestimates urinary immunoglobulin light chains when albumin or pooled serum is used as the protein standard. The specific color yields of these and other proteins can be brought closer together by
Germán L Rosano et al.
The Journal of biological chemistry, 286(34), 29671-29680 (2011-07-09)
HSP100 proteins are molecular chaperones involved in protein quality control. They assist in protein (un)folding, prevent aggregation, and are thought to participate in precursor translocation across membranes. Caseinolytic proteins ClpC and ClpD from plant chloroplasts belong to the HSP100 family.
Qi Zong Lao et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 24(12), 5013-5023 (2010-08-25)
Voltage-gated calcium channels conduct Ca(2+) ions in response to membrane depolarization. The resulting transient increase in cytoplasmic free calcium concentration is a critical trigger for the initiation of such vital responses as muscle contraction and transcription. L-type Ca(v)1.2 calcium channels
Lei Yue et al.
Nucleic acids research, 48(17), 9589-9605 (2020-08-29)
Transcription termination defines accurate transcript 3'-ends and ensures programmed transcriptomes, making it critical to life. However, transcription termination mechanisms remain largely unknown in Archaea. Here, we reported the physiological significance of the newly identified general transcription termination factor of Archaea
Gel-filtration chromatography
F'ag'ain 'O?C, et al.
Journal of biological and chemical chronicles, 25-33 (2011)
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