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Key Documents

I5029

Sigma-Aldrich

IgG1, Lambda from human myeloma plasma

purified immunoglobulin, >95% (SDS-PAGE)

Synonyme(s) :

Human IgG1-λ

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

human

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Pureté

>95% (SDS-PAGE)

Conditions d'expédition

dry ice

Température de stockage

−20°C

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Description générale

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG1 is the most abundant of the IgG subclass
Human myeloma IgG1, λ is purified from human plasma by fractionation, ion-exchange, and affinity chromatography procedures.
IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane. IgG is further subdivided into four subclass- IgG1, IgG2, IgG3 and IgG4.Elevation in antigen specific IgG1 is associated with symptomatic infection and Th2-regulation. IgG1 λ from human myeloma can be used in quantitative ELISA.Human IgG1 λ react specifically with human IgG.

Application

IgG1 λ from human myeloma can be used as reference antigen, blocking agent, immunoglobulin calibrator or coating protein in various immunoassay like ELISA, dot blot immunobinding, immunodiffusion, immunoelectrophoresis, hemagglutination, cell-binding assays and western immunoblotting.
The purified IgG1, λ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Human myeloma IgG1, λ was used as non-specific control in quantitative ELISA and solid-phase ELISA. A range of concentrations of IgG1, λ from 10 to 100 ng was used for quantitative ELISA. Human myeloma IgG1, λ was also used as control in size-exclusion chromatography to analyse purity of recombinant antibodies.

Forme physique

Solution in 0.02 M Tris buffered saline, pH 8.0.

Remarque sur l'analyse

Identity is determined by immunoelectrophoresis and indirect ELISA.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

M J Day
Veterinary parasitology, 147(1-2), 2-8 (2007-05-01)
Infection with Leishmania may have different outcomes in genetically distinct individuals and the course of infection is determined by the nature of the host innate and adaptive immune response. Thus in experimentally infected mice, and in naturally infected dogs or
Cristina Capodicasa et al.
Plant biotechnology journal, 9(7), 776-787 (2011-01-27)
There is an increasing interest in the development of therapeutic antibodies (Ab) to improve the control of fungal pathogens, but none of these reagents is available for clinical use. We previously described a murine monoclonal antibody (mAb 2G8) targeting β-glucan
W L Zhao et al.
Journal of the European Academy of Dermatology and Venereology : JEADV, 36(2), 271-278 (2021-10-28)
The detection of serum anti-desmoglein (Dsg) IgG autoantibodies has been reported to be useful for assessment of disease activity in pemphigus. However, previous studies have reported that anti-Dsg autoantibodies remain detectable in some patients without active pemphigus lesions. To investigate
Targeting CD70 in combination with chemotherapy to enhance the anti-tumor immune effects in non-small cell lung cancer.
Flieswasser, et al.
Oncoimmunology, 12, 2192100-2192100 (2023)
A M Lew
Journal of immunological methods, 72(1), 171-176 (1984-08-03)
The antibody titre and the optical density values in an ELISA are influenced by the epitope density of the antigen and the affinity of the antibody tested. This has major implications in the interpretation of ELISA results.

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