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Merck
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H9914

Millipore

HIS-Select® Nickel Magnetic Agarose Beads

Synonyme(s) :

nickel charged magnetic beaded agarose

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

Conjugué

magnetic beads

Forme

suspension

Durée de conservation

2 yr (Unopened product)

Matrice

6% beaded magnetic agarose

Capacité

≥15 mg/mL binding capacity

Température de stockage

2-8°C

Description générale

HIS-Select Magnetic Agarose Beads consist of paramagnetic, immobilized metal-ion affinity chromatography (IMAC) resin that contain a proprietary quadridentate chelate, which is bound with nickel and covalently attached through a non-charged, hydrophilic linker to magnetic beaded agarose. The magnetic properties of the beads aid in manipulations, such as repetitive washings, and recovery of the protein bound beads. This leads to greater experimental reproducibility and more accurate quantitation of the His-tagged proteins of interest.

Application

The HIS-Select Nickel Magnetic Agarose Beads are designed for use in automated and small-scale affinity capture (molecular pull-down) purifications of histidine-tagged protein / His-tag protein while exhibiting low non-specific binding of other proteins. The beads can be used to purify His-tagged proteins under native and denaturing conditions.

Forme physique

Supplied as a 50% slurry suspension in 30% Ethanol.

Informations légales

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

08168

Timestrip Plus™ 8 °C

Pictogrammes

FlameHealth hazardExclamation mark
GHS02,GHS08,GHS07

Mention d'avertissement

Danger

Classification des risques

Aquatic Chronic 3 - Carc. 1B - Flam. Liq. 3 - Repr. 1B - Skin Sens. 1 - STOT RE 2

Organes cibles

Respiratory Tract

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

86.0 °F

Point d'éclair (°C)

30 °C

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Certificats d'analyse (COA)

Lot/Batch Number
0000362903
0000330053
0000362903
0000330053
SLCN0486

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Sonja Hänzelmann et al.
Clinical epigenetics, 7, 19-19 (2015-03-13)
Primary cells enter replicative senescence after a limited number of cell divisions. This process needs to be considered in cell culture experiments, and it is particularly important for regenerative medicine. Replicative senescence is associated with reproducible changes in DNA methylation
Mei Suen Kong et al.
Science signaling, 12(567) (2019-02-07)
T cell activation is initiated by signaling molecules downstream of the T cell receptor (TCR) that are organized by adaptor proteins. CIN85 (Cbl-interacting protein of 85 kDa) is one such adaptor protein. Here, we showed that CIN85 limited T cell
Philip Vitorino et al.
Nature, 519(7544), 425-430 (2015-03-25)
Cell migration is a stepwise process that coordinates multiple molecular machineries. Using in vitro angiogenesis screens with short interfering RNA and chemical inhibitors, we define here a MAP4K4-moesin-talin-β1-integrin molecular pathway that promotes efficient plasma membrane retraction during endothelial cell migration.
Joshua W Burgess et al.
American journal of respiratory cell and molecular biology, 41(6), 714-721 (2009-03-17)
Pneumocystis organisms are opportunistic fungal pathogens that cause significant pneumonia in immune-compromised hosts. Recent evidence has suggested that Pneumocystis carinii exists as separate mating types, and expresses and regulates proteins that govern meiosis and progression of the life cycle. This
Yun Wei et al.
Journal of materials chemistry. B, 1(15), 2066-2071 (2013-04-21)
The imidazolium cation was chosen as protein selective affinity group and a kind of ionic liquid magnetic microspheres was developed by immobilizing imidazolium cations onto the surface of silica-coated magnetic microspheres to form imidazolium ionic liquid modified magnetic microspheres (Fe3O4@SiO2@IL).
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Contenu apparenté

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Purification des protéines

Techniques, réactifs et protocoles permettant de purifier les protéines recombinantes par différentes méthodes telles que la chromatographie d'échange d'ions, la chromatographie d'exclusion stérique ou la chromatographie d'affinité.

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

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