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GE17-6003-19

DeStreak Rehydration Solution

Cytiva 17-6003-19, pkg of 5 × 3 mL

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About This Item

Code UNSPSC :
41105323
Nomenclature NACRES :
NA.25

Forme

liquid

Caractéristiques

wetted part: no

Conditionnement

pkg of 5 × 3 mL

Fabricant/nom de marque

Cytiva 17-6003-19

Conditions de stockage

(Rehydration Solution -20 °C Reagent 2 - 8 °C)

Couleur

Colorless

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

DeStreak Rehydration Solution.

Application

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsen the problem. Extra spots on 2-D gels, caused by nonspecific oxidation of Proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and nonspecific oxidation result in poorly resolved Protein patterns and reduced reproducibility between electrophoresis runs.

DeStreak Rehydration Solution effectively eliminates nonspecific oxidation by maintaining Protein thiol groups in a single oxidation state, irrespective of sample load, pH range, or run length. Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis. The effectiveness of DeStreak Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis.

DeStreak Rehydration Solution contains optimized concentrations of urea, thiourea, CHAPS, and DeStreak Reagent. The solution is ready for use after addition of the appropriate IPG buffer. DeStreak Reagent is also available separately in a 1-ml pack size.

Caractéristiques et avantages

  • Improves reproducibility and quality of 2-D gels by preventing streaking.
  • Eliminates extra spots caused by nonspecific oxidation of Proteins.
  • Stabilizes Proteins throughout entire 2-D runs, ensuring simplified, well-resolved Protein patterns.
  • Highly suitable for both analytical and preparative gels.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

DeStreak is a trademark of Cytiva

Pictogrammes

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Mention d'avertissement

Warning

Mentions de danger

Code de la classe de stockage

12 - Non Combustible Liquids


Certificats d'analyse (COA)

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Articles

This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.

Protocoles

This protocol shows how to prepare the Immobiline® DryStrip Kit and electrodes strips.

This page shows how to apply higher sample volumes and protein amounts with paper bridges in isoelectric focusing with Cytiva products

Troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from Cytiva.

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