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D2196

Sigma-Aldrich

DAF-FM solution

5 mM in DMSO, ≥97% (HPLC)

Synonyme(s) :

4-Amino-5-methylamino- 2′,7′-difluorofluorescein, Diaminofluorescein-FM

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About This Item

Formule empirique (notation de Hill):
C21H14F2N2O5
Numéro CAS:
254109-20-1
Poids moléculaire :
412.34
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77

Niveau de qualité

100

Pureté

≥97% (HPLC)

Forme

DMSO solution

Conditions de stockage

desiccated

Couleur

colorless to yellow, including pink

Conditions d'expédition

wet ice

Température de stockage

−20°C

InChI

1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3

Clé InChI

DIJCILWNOLHJCG-UHFFFAOYSA-N

Application

DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples.

Actions biochimiques/physiologiques

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm; Em: 515 nm; dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.

Caractéristiques et avantages

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Detection of protein S-nitrosothiols (SNOs) in plant samples on diaminofluorescein (DAF) gels
Rodriguez-Ruiz M, et al.
Bio-protocol null
Marta Rodríguez-Ruiz et al.
Bio-protocol, 7(18), e2559-e2559 (2017-09-20)
In plant cells, the analysis of protein S-nitrosothiols (SNOs) under physiological and adverse stress conditions is essential to understand the mechanisms of Nitric oxide (NO)-based signaling. We adapted a previously reported protocol for detecting protein SNOs in animal systems (

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