C7438

Chaperonin 10 from Escherichia coli

≥95.0% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder

• Synonyme(s) : GroES
• Numéro MDL: MFCD00163256
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: Escherichia coli
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli overproducing strain
• Pureté: ≥95.0% (SDS-PAGE)
• Forme: lyophilized powder
• Technique(s): activity assay: suitable
• Numéro d'accès UniProt: P61604
• Température de stockage: 2-8°C
• Informations sur le gène: human ... HSPE1(3336)

Description

Actions biochimiques/physiologiques

Chaperonin60 (GroEL) and chaperonin10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles. The chaperonins assist the folding of nascent, organelle-imported or stress-destabilized polypeptides. In vitro, purified GroEL together with purified GroES in the presence of Mg-ATP facilitate refolding and reactivation of denatured proteins, e.g., the photosynthetic enzyme rubisco and the mitochondrial enzyme rhodanese.
The folding activity of a 1:1 molar mixture of GroEL and GroES was tested using urea-denatured rhodanese. At least 2-fold reactivation of rhodanese over the spontaneous reactivation was obtained.

Conditionnement

Package size based on protein content.

Forme physique

Lyophilized powder containing Tris buffer salts, potassium chloride, dithiothreitol and trehalose as stabilizer.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)